Overview

  • Product name

    Anti-CD68 antibody [Y1/82A] (FITC)
    See all CD68 primary antibodies
  • Description

    Mouse monoclonal [Y1/82A] to CD68 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Normal lymphocytes stimulated with phytohaemagluttin/Freund's adjuvant

  • Positive control

    • Human peripheral blood monocytes for Flow cytometry.
  • General notes

    The reagent is free of unconjugated FITC.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 98% PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Purified by affinity chromatography. Purity of this monoclonal antibodies is 95%.
  • Clonality

    Monoclonal
  • Clone number

    Y1/82A
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5-20µl for 106 cells.

in 100 µl staining volume. Or Use 5 - 20 µl for 100 µl of whole blood.

 

 

 

ab18427 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Intracellular immunofluorescent staining with flow cytometric analysis labelling CD68 in Human peripheral blood monocytes intracellularly stained by ab134351.

References

This product has been referenced in:

See 1 Publication for this product

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