Recombinant
RabMAb

Recombinant Anti-CD69 antibody [EPR21811] (ab233395)

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Overview

  • Product name

    Anti-CD69 antibody [EPR21811]
    See all CD69 primary antibodies

  • Description

    Rabbit monoclonal [EPR21811] to CD69

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD69 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q07108

  • Positive control

    • WB: Daudi treated with 50ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours whole cell lysate; H9 whole cell lysate. IHC-P: Human tonsil and cervix cancer tissues.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24-32 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

  • Tissue specificity

    Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Developmental stage

    Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

  • Post-translational
    modifications

    Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activation inducer molecule (AIM/CD69) antibody
      • Activation inducer molecule antibody
      • AIM antibody
      • BL-AC/P26 antibody
      • BLAC/P26 antibody
      • C-type lectin domain family 2 member C antibody
      • CD69 antibody
      • CD69 antigen (p60, early T-cell activation antigen) antibody
      • CD69 antigen antibody
      • CD69 molecule antibody
      • CD69_HUMAN antibody
      • CLEC2C antibody
      • EA1 antibody
      • Early activation antigen CD69 antibody
      • Early lymphocyte activation antigen antibody
      • Early T cell activation antigen p60 antibody
      • Early T-cell activation antigen p60 antibody
      • GP32/28 antibody
      • Leu23 antibody
      • Leukocyte surface antigen Leu-23 antibody
      • MLR-3 antibody
      • MLR3 antibody
      • VEA antibody
      • Very Early Activation Antigen antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD69 antibody [EPR21811] (ab233395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD69 antibody [EPR21811] (ab233395)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD69 with ab233395 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in lymphoid cells of human tonsil (PMID: 2807372). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-CD69 antibody [EPR21811] (ab233395)
      Western blot - Anti-CD69 antibody [EPR21811] (ab233395)
      All lanes : Anti-CD69 antibody [EPR21811] (ab233395) at 1/1000 dilution

      Lane 1 : Un-treated Daudi (human Burkitt's lymphoma cell line) whole cell lysate
      Lane 2 : Daudi, treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa
      Observed band size: 28,32 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      PMA treatment increases the basal level of p28/32 in Daudi cells (PMID: 1617156).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD69 antibody [EPR21811] (ab233395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD69 antibody [EPR21811] (ab233395)

      Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling CD69 with ab233395 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in infiltrated lymphoid cells of human cervical cancer is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Western blot - Anti-CD69 antibody [EPR21811] (ab233395)
      Western blot - Anti-CD69 antibody [EPR21811] (ab233395)
      Anti-CD69 antibody [EPR21811] (ab233395) at 1/1000 dilution + H9 (human cutaneous T lymphocyte lymphoma cell line) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa
      Observed band size: 24,27 kDa why is the actual band size different from the predicted?


      Exposure time: 6 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      A distinct band with an apparent molecular mass of 24 kDa and a fainter band with an apparent molecular mass of 27kDa were observed (PMID: 2784767).

    References

    ab233395 has not yet been referenced specifically in any publications.

    Publishing research using ab233395? Please let us know so that we can cite the reference in this datasheet.

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