Anti-CD69 antibody [H1.2F3] (ab25190)
Key features and details
- Armenian Hamster monoclonal [H1.2F3] to CD69
- Suitable for: IHC-FoFr, Flow Cyt, IP, Cellular Activation
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-CD69 antibody [H1.2F3]
See all CD69 primary antibodies -
Description
Armenian Hamster monoclonal [H1.2F3] to CD69 -
Host species
Armenian hamster -
Specificity
ab25190 recognises mouse CD69/Very Early Activation Antigen (VEA),. -
Tested applications
Suitable for: IHC-FoFr, Flow Cyt, IP, Cellular Activationmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue/ cell preparation (Mouse) - Y245 mouse dendritic epidermal T cell line.
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General notes
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.2
Constituent: 100% Borate buffered saline -
Concentration information loading...
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Purity
Affinity purified -
Primary antibody notes
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells. -
Clonality
Monoclonal -
Clone number
H1.2F3 -
Isotype
IgG -
Light chain type
lambda -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 22483694
|
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Flow Cyt |
Use at an assay dependent concentration.
Pubmed:2838547 and Pubmed:2026886. ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP |
Use at an assay dependent concentration.
Please see: Pubmed:2838547. |
|
Cellular Activation |
Use at an assay dependent concentration.
T cell and NK cells. Please see: Pubmed:2838547 and Pubmed:2026886. |
Notes |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 22483694 |
Flow Cyt
Use at an assay dependent concentration. Pubmed:2838547 and Pubmed:2026886. ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. Please see: Pubmed:2838547. |
Cellular Activation
Use at an assay dependent concentration. T cell and NK cells. Please see: Pubmed:2838547 and Pubmed:2026886. |
Target
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Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. -
Tissue specificity
Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
Earliest inducible cell surface glycoprotein acquired during lymphoid activation. -
Post-translational
modificationsConstitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12515 Mouse
- SwissProt: P37217 Mouse
- Unigene: 74745 Mouse
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Alternative names
- Activation inducer molecule (AIM/CD69) antibody
- Activation inducer molecule antibody
- AIM antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab25190 has been referenced in 2 publications.
- Aftabizadeh M et al. Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis. JCI Insight 6:N/A (2021). PubMed: 33491667
- Ishizaki S et al. Role of CD69 in acute lung injury. Life Sci 90:657-65 (2012). IHC-FoFr ; Mouse . PubMed: 22483694