Overview

  • Product name

    Anti-CD6/T12 antibody [5A10A2]
    See all CD6/T12 primary antibodies
  • Description

    Mouse monoclonal [5A10A2] to CD6/T12
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD6/T12 aa 18-199. Expressed in E. Coli.
    Sequence:

    HPS PAPPDQLNTS SAESELWEPG ERLPVRLTNG SSSCSGTVEV RLEASWEPAC GALWDSRAAE AVCRALGCGG AEAASQLAPP TPELPPPPAA GNTSVAANAT LAGAPALLCS GAEWRLCEVV EHACRSDGRR ARVTCAENRA LRLVDGGGAC AGRVEMLEHG EWGSVCDDTW DLEDAHVVC


    Database link: P30203

  • Positive control

    • Recombinant Human CD6/T12 (aa18-199) recombinant protein; CD6/T12 (aa 18-199)-hIgGFc transfected HEK293 cell lysate.
  • General notes

    Previously labelled as CD6. 

Properties

Applications

Our Abpromise guarantee covers the use of ab204949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.
WB 1/500 - 1/2000. Predicted molecular weight: 72 kDa.
IHC-P 1/200 - 1/1000.

Target

  • Function

    Involved in cell adhesion. Binds to CD166.
  • Tissue specificity

    Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
  • Sequence similarities

    Contains 3 SRCR domains.
  • Post-translational
    modifications

    After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
    Contains intrachain disulfide bond(s).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD_antigen=CD6 antibody
    • CD6 antibody
    • CD6 antigen Tp120 antibody
    • CD6 molecule antibody
    • CD6_HUMAN antibody
    • FLJ44171 antibody
    • OX52 antibody
    • T cell differentiation antigen CD6 antibody
    • T-cell differentiation antigen CD6 antibody
    • T12 antibody
    • TP120 antibody
    see all

Images

  • All lanes : Anti-CD6/T12 antibody [5A10A2] (ab204949) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : CD6/T12 (aa 18-199)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 72 kDa

  • Flow cytometric analysis of Jurkat cells labeling CD6/T12 with ab204949 at 1/200 dilution (green) compared with a negative control (red).

  • Anti-CD6/T12 antibody [5A10A2] (ab204949) at 1/500 dilution + Human CD6/T12 (aa: 18-199) recombinant protein

    Predicted band size: 72 kDa

References

ab204949 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204949.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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