Anti-CD6/T12 antibody [C6/372 + 3F7B5] (ab199008)

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Overview

  • Product name

    Anti-CD6/T12 antibody [C6/372 + 3F7B5]
    See all CD6/T12 primary antibodies

  • Description

    Mouse monoclonal [C6/372 + 3F7B5] to CD6/T12

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, IP, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This product was produced with the following immunogens:
    Recombinant full length protein corresponding to Human CD6/T12 aa 1-668. (C6/372)
    Sequence:

    MWLFFGITGLLTAALSGHPSPAPPDQLNTSSAESELWEPGERLPVRLTNG SSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPP TPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRR ARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCR QLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCG HKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAK VLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLC SQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESRELM LLIPSIVLGILLLGSLIFIAFILLRIKGKYALPVMVNHQHLPTTIPAGSN SYQPVPITIPKEVFMLPIQVQAPPPEDSDSGSDSDYEHYDFSAQPPVALT TFYNSQRHRVTDEEVQQSRFQMPPLEEGLEELHASHIPTANPGHCITDPP SLGPQYHPRSNSESSTSSGEDYCNSPKSKLPPWNPQVFSSERSSFLEQPP NLELAGTQPAFSAGPPADDSSSTSSGEWYQNFQPPPQPPSEEQFGCPGSP SPQPDSTDNDDYDDISAA


    Database link: P30203

    Tissue, cells or virus corresponding to Human CD6/T12. Human rheumatoid synovial T cell line ST-1 (3F7B5).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human tonsil tissue. CCRF-CEM. Jurkat cells.

  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    Previously labelled as CD6. 

Properties

Applications

Our Abpromise guarantee covers the use of ab199008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 72 kDa.
IP Use at 1-2 µg/mg of lysate.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Involved in cell adhesion. Binds to CD166.

  • Tissue specificity

    Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

  • Sequence similarities

    Contains 3 SRCR domains.

  • Post-translational
    modifications

    After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
    Contains intrachain disulfide bond(s).

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD_antigen=CD6 antibody
      • CD6 antibody
      • CD6 antigen Tp120 antibody
      • CD6 molecule antibody
      • CD6_HUMAN antibody
      • FLJ44171 antibody
      • OX52 antibody
      • T cell differentiation antigen CD6 antibody
      • T-cell differentiation antigen CD6 antibody
      • T12 antibody
      • TP120 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/372 + 3F7B5] (ab199008)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/372 + 3F7B5] (ab199008)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD6/T12 with ab199008 at 1 µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/372 + 3F7B5] (ab199008)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/372 + 3F7B5] (ab199008)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD6/T12 with ab199008 at 1 µg/ml.

    References

    ab199008 has not yet been referenced specifically in any publications.

    Publishing research using ab199008? Please let us know so that we can cite the reference in this datasheet.

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