Product nameAnti-CD6/T12 antibody [C6/372 + 3F7B5]
See all CD6/T12 primary antibodies
DescriptionMouse monoclonal [C6/372 + 3F7B5] to CD6/T12
Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, IP, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Human CD6/T12 aa 1-668. (C6/372)
MWLFFGITGLLTAALSGHPSPAPPDQLNTSSAESELWEPGERLPVRLTNG SSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPP TPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRR ARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCR QLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCG HKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAK VLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLC SQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESRELM LLIPSIVLGILLLGSLIFIAFILLRIKGKYALPVMVNHQHLPTTIPAGSN SYQPVPITIPKEVFMLPIQVQAPPPEDSDSGSDSDYEHYDFSAQPPVALT TFYNSQRHRVTDEEVQQSRFQMPPLEEGLEELHASHIPTANPGHCITDPP SLGPQYHPRSNSESSTSSGEDYCNSPKSKLPPWNPQVFSSERSSFLEQPP NLELAGTQPAFSAGPPADDSSSTSSGEWYQNFQPPPQPPSEEQFGCPGSP SPQPDSTDNDDYDDISAA
Database link: P30203
Tissue, cells or virus corresponding to Human CD6/T12. Human rheumatoid synovial T cell line ST-1 (3F7B5).
- Human tonsil tissue. CCRF-CEM. Jurkat cells.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Previously labelled as CD6.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Clone numberC6/372 + 3F7B5
Our Abpromise guarantee covers the use of ab199008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 72 kDa.|
|IP||Use at 1-2 µg/mg of lysate.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
FunctionInvolved in cell adhesion. Binds to CD166.
Tissue specificityExpressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
Sequence similaritiesContains 3 SRCR domains.
modificationsAfter T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Contains intrachain disulfide bond(s).
- Information by UniProt
- CD_antigen=CD6 antibody
- CD6 antibody
- CD6 antigen Tp120 antibody
ab199008 has not yet been referenced specifically in any publications.