Product nameAnti-CD6/T12 antibody [EPR4057]
See all CD6/T12 primary antibodies
DescriptionRabbit monoclonal [EPR4057] to CD6/T12
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human CD6/T12 aa 550-650. (The amino acid sequence is considered to be commercially sensitive)
Database link: P30203
- WB: Jurkat, Human tonsil, PBMC and HuT-78 lysates IHC-P: Human tonsil and Human lymphocytes infiltrating colon tissues
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as CD6.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 72 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Antigen retrieval is recommended.|
|ICC||1/100 - 1/250.|
FunctionInvolved in cell adhesion. Binds to CD166.
Tissue specificityExpressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
Sequence similaritiesContains 3 SRCR domains.
modificationsAfter T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Contains intrachain disulfide bond(s).
- Information by UniProt
- CD_antigen=CD6 antibody
- CD6 antibody
- CD6 antigen Tp120 antibody
All lanes : Anti-CD6/T12 antibody [EPR4057] (ab109217) at 1/1000 dilution
Lane 1 : Jurkat lysates
Lane 2 : Human tonsil lysates
Lane 3 : PBMC lysates
Lane 4 : HuT-78 lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 90-130 kDa why is the actual band size different from the predicted?
ab109217, at a 1/100 dilution, staining CD6/T12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
ab109217, at a 1/100 dilution, staining CD6/T12 in paraffin embedded Human lymphocytes infiltrating colon tissue by Immunohistochemistry.
ab109217 showing negative staining in Skeletal muscle tissue.
ab109217 showing negative staining in Normal brain tissue.
ab109217 showing negative staining in Normal kidney tissue.
ab109217 showing negative staining in Normal uterus tissue.
ab109217 has not yet been referenced specifically in any publications.