Recombinant Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4057] to CD6/T12 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free
See all CD6/T12 primary antibodies -
Description
Rabbit monoclonal [EPR4057] to CD6/T12 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HuT-78, and Human peripheral blood mononuclear cells whole cell lysates. Human tonsil tissue lysate; IP: Human peripheral blood mononuclear cells whole cell lysate; ICC/IF: Human peripheral blood mononuclear cells; IHC-P: Human tonsil and Human lymphocytes infiltrating colon tissues; Flow Cyt (intra): HuT-78 cells.
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General notes
ab247795 is the carrier-free version of ab109217.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4057 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD6/T12 antibody [EPR4057] (ab109217)
- PE Anti-CD6 / T12 antibody [EPR4057] (ab306500)
- APC Anti-CD6 / T12 antibody [EPR4057] (ab306501)
- HRP Anti-CD6 / T12 antibody [EPR4057] (ab306502)
- Alexa Fluor® 488 Anti-CD6 / T12 antibody [EPR4057] (ab309736)
- Alexa Fluor® 647 Anti-CD6 / T12 antibody [EPR4057] (ab310102)
- Alexa Fluor® 594 Anti-CD6 / T12 antibody [EPR4057] (ab310507)
- Alexa Fluor® 555 Anti-CD6 / T12 antibody [EPR4057] (ab312036)
- Alexa Fluor® 568 Anti-CD6 / T12 antibody [EPR4057] (ab312513)
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 90-130 kDa (predicted molecular weight: 72 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 90-130 kDa (predicted molecular weight: 72 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Involved in cell adhesion. Binds to CD166. -
Tissue specificity
Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. -
Sequence similarities
Contains 3 SRCR domains. -
Post-translational
modificationsAfter T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Contains intrachain disulfide bond(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 923 Human
- Omim: 186720 Human
- SwissProt: P30203 Human
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Alternative names
- CD_antigen=CD6 antibody
- CD6 antibody
- CD6 antigen Tp120 antibody
see all
Images
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Anti-CD6/T12 antibody [EPR4057] (ab109217) at 1/5000 dilution (Purified) + HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 105-130 kDa why is the actual band size different from the predicted?The molecular weight of CD6 ranges from 105 to 130 kDa depending on its degree of phosphorylation (PMID: 17601777).
Blocking/Diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109217).
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Immunocytochemistry/ Immunofluorescence analysis of Human PBMC (Human primary peripheral blood mononuclear cell) cells labeling CD6/T12 with Purified ab109217 at 1:50 dilution (2.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109217).
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Intracellular Flow Cytometry analysis of HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) cells labeling CD6/T12 with Purified ab109217 at 1/20 dilution (10µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109217).
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Purified ab109217 at 1:20 dilution (0.6μg) immunoprecipitating CD6/T12 in HuT-78 whole cell lysate.
Lane 1 (input): HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10μg
Lane 2 (+): ab109217 + HuT-78 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109217 in HuT-78 whole cell lysate.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 105-130 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109217).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD6/T12 with purified ab109217 at 1/1000 dilution (0.115 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109217).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247795 has not yet been referenced specifically in any publications.