Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab130145 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab130145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 25 kDa).|
FunctionNot yet known.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
- Information by UniProt
- CD7 antibody
- CD7 antigen (p41) antibody
- CD7 antigen antibody
Anti-CD7 antibody (ab130145) at 0.5 µg/ml + Human Thymus lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. We do note that CD7 is a glycoprotein which makes a higher size band to be expected. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
ab130145 has not yet been referenced specifically in any publications.