Overview

  • Product name

  • Description

    Goat polyclonal to CD7
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide:

    TEEQSQGWHRCSDAP

    , corresponding to internal sequence amino acids 132-146 of Human CD7 (NP_006128.1).
    The immunizing peptide corresponds to a part of the extracellular domain.

  • Positive control

    • Human Thymus lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab130145 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    Not yet known.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD7 antibody
    • CD7 antigen (p41) antibody
    • CD7 antigen antibody
    • CD7 molecule antibody
    • CD7_HUMAN antibody
    • GP40 antibody
    • LEU 9 antibody
    • LEU9 antibody
    • p41 protein antibody
    • T cell antigen CD7 antibody
    • T cell leukemia antigen antibody
    • T cell surface antigen Leu 9 antibody
    • T-cell antigen CD7 antibody
    • T-cell leukemia antigen antibody
    • T-cell surface antigen Leu-9 antibody
    • Tp 40 antibody
    • Tp40 antibody
    • TP41 antibody
    see all

Images

  • Anti-CD7 antibody (ab130145) at 0.5 µg/ml + Human Thymus lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

    We do note that CD7 is a glycoprotein which makes a higher size band to be expected. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.

References

ab130145 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab130145.
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