Recombinant
RabMAb

Recombinant Anti-CD7 antibody [EPR22065] (ab224194)

Overview

  • Product name

    Anti-CD7 antibody [EPR22065]
    See all CD7 primary antibodies
  • Description

    Rabbit monoclonal [EPR22065] to CD7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD7 aa 1-200. The exact sequence is proprietary.
    Database link: P09564

  • Positive control

    • WB: MOLT-4 and HepG2 whole cell lysates; Human tonsil and thymus lysates. IP: Human tonsil lysate. ICC/IF: MOLT-4 cells. Flow cyt: Human PBMCs.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22065
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/100.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Not yet known.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD7 antibody
    • CD7 antigen (p41) antibody
    • CD7 antigen antibody
    • CD7 molecule antibody
    • CD7_HUMAN antibody
    • GP40 antibody
    • LEU 9 antibody
    • LEU9 antibody
    • p41 protein antibody
    • T cell antigen CD7 antibody
    • T cell leukemia antigen antibody
    • T cell surface antigen Leu 9 antibody
    • T-cell antigen CD7 antibody
    • T-cell leukemia antigen antibody
    • T-cell surface antigen Leu-9 antibody
    • Tp 40 antibody
    • Tp40 antibody
    • TP41 antibody
    see all

Images

  • Human PBMCs (peripheral blood mononuclear cells) were stained with Alexa Fluor® 647-conjugated anti-human CD3 and rabbit IgG - ab172730 (Left) or CD7 - ab224194 at 1/500 (Right). Goat anti-Rabbit IgG Fc (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody. Data shown was gated on viable cells. The expression pattern of CD7 is consistent with literature. (PMID: 3260936)

  • All lanes : Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times:

    Lane 1: 3 minutes

    Lane 2: 48 seconds

    This blot was developed using a higher sensitivity ECL substrate.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 24157461, PMID: 2479685); the actual size different from the predicted is due to glycosylation (PMID: 2479685).

     

     

  • All lanes : Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

    Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 25 kDa
    Additional bands at: 37 kDa (possible non-specific secondary antibody binding)


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 24157461, PMID: 2479685); the actual size different from the predicted is due to glycosylation (PMID: 2479685).

    Negative control: Raji (PMID: 1633296, PMID: 11389079).

     

  • CD7 was immunoprecipitated from 0.35 mg of human tonsil lysate with ab224194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224194 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human tonsil lysate 10 μg (Input).
    Lane 2: ab224194 IP in human tonsil lysate lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224194 in human tonsil lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD7 with ab224194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in MOLT-4 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PDS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: Raji (PMID: 16332967).

References

ab224194 has not yet been referenced specifically in any publications.

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