Overview

  • Product name
    Anti-CD7 antibody [EPR4242]
    See all CD7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4242] to CD7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD7 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Human tonsil, spleen and peripheral blood mononuclear cell (PBMC) lysates IHC-P: Human tonsil and colonic tissues ICC/IF: Jurkat cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 2.40 x 10 -12 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4242
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 25 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Not yet known.
    • Sequence similarities
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD7 antibody
      • CD7 antigen (p41) antibody
      • CD7 antigen antibody
      • CD7 molecule antibody
      • CD7_HUMAN antibody
      • GP40 antibody
      • LEU 9 antibody
      • LEU9 antibody
      • p41 protein antibody
      • T cell antigen CD7 antibody
      • T cell leukemia antigen antibody
      • T cell surface antigen Leu 9 antibody
      • T-cell antigen CD7 antibody
      • T-cell leukemia antigen antibody
      • T-cell surface antigen Leu-9 antibody
      • Tp 40 antibody
      • Tp40 antibody
      • TP41 antibody
      see all

    Images

    • All lanes : Anti-CD7 antibody [EPR4242] (ab109296) at 1/1000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 25 kDa
      Observed band size: 37 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemical analysis of CD7 in paraffin embedded Human tonsil tissue, using ab109296 at a diluiton of 1/100.
    • Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD7 with Ab109296 at 1/250. ab150077 AlexaFluor®488 Goat anti-Rabbit weas used as a the secondary antibody at 1/1000. Cells were fixed with 100% methanol. Nuclei are stained blue with DAPI. 

      Confocal image showing membranous staining on Jurkat cell line.

    • Immunohistochemical analysis of CD7 in paraffin embedded Human colonic tissue, using ab109296 at a diluiton of 1/100.
    • Anti-CD7 antibody [EPR4242] (ab109296) at 1/1000 dilution + Human peripheral blood mononuclear cell (PBMC) lysate at 10 µg

      Predicted band size: 25 kDa
      Observed band size: 37 kDa (why is the actual band size different from the predicted?)

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Ng SY  et al. Targetable vulnerabilities in T- and NK-cell lymphomas identified through preclinical models. Nat Commun 9:2024 (2018). Read more (PubMed: 29789628) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109296.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up