Overview

  • Product name

    Anti-CD7 antibody [SP94]
    See all CD7 primary antibodies
  • Description

    Rabbit monoclonal [SP94] to CD7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD7 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P09564

  • Positive control

    • Human tonsil, Human thymus, Human colon, Human B cell lymphoma, Human reactive lymph node, Human skin squamous cell carcinoma, Human renal cell carcinoma, Human stomach, Human stomach adenocarcinoma and Human spleen tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SP94
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.

Antigen Retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function

    Not yet known.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD7 antibody
    • CD7 antigen (p41) antibody
    • CD7 antigen antibody
    • CD7 molecule antibody
    • CD7_HUMAN antibody
    • GP40 antibody
    • LEU 9 antibody
    • LEU9 antibody
    • p41 protein antibody
    • T cell antigen CD7 antibody
    • T cell leukemia antigen antibody
    • T cell surface antigen Leu 9 antibody
    • T-cell antigen CD7 antibody
    • T-cell leukemia antigen antibody
    • T-cell surface antigen Leu-9 antibody
    • Tp 40 antibody
    • Tp40 antibody
    • TP41 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling CD7 with ab183325 at 1/50 dilution (2.0 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells in human stomach carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183325 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD7 with ab183325 at 1: 50 dilution (2.0 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183325 for 30 mins at room temperature.

  • Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human stomach adenocarcinoma tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human renal cell carcinoma tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human reactive lymph node tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD7 with ab183325 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD7 with ab183325 at 1/50.

References

ab183325 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183325.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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