Key features and details
- Rabbit polyclonal to CD70
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CD70 antibody
See all CD70 primary antibodies
DescriptionRabbit polyclonal to CD70
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human CD70 aa 1-193.
MPEEGSGCSVRRRPYGCVLRAALVPLVAGLVICLVVCIQRFAQAQQQLPL ESLGWDVAELQLNHTGPQQDPRLYWQGGPALGRSFLHGPELDKGQLRIHR DGIYMVHIQVTLAICSSTTASRHHPTTLAVGICSPASRSISLLRLSFHQG CTIASQRLTPLARGDTLCTNLTGTLLPSRNTDETFFGVQWVRP
Database link: P32970
- WB: HL-60 cell lysate and Mouse liver tissue lysate ICC/IF: U-2 OS cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 21 kDa.|
FunctionCytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- CD 27L antibody
- CD 70 antibody
- CD27 L antibody
Immunofluorescence staining of U-2 OS cells stained for CD70 with ab175389. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-CD70 antibody (ab175389) at 1/1000 dilution
Lane 1 : HL-60 cell lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat AntiRabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 21 kDa
Blocking buffer: 3% nonfat dry milk in TBST
ab175389 has been referenced in 2 publications.
- Zheng M et al. Efficacy of B7-H3-Redirected BiTE and CAR-T Immunotherapies Against Extranodal Nasal Natural Killer/T Cell Lymphoma. Transl Oncol 13:100770 (2020). PubMed: 32298986
- Knox T et al. Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells. Sci Rep 9:6136 (2019). PubMed: 30992475