Overview

  • Product name

    Anti-CD70 antibody [BU69]
    See all CD70 primary antibodies
  • Description

    Mouse monoclonal [BU69] to CD70
  • Host species

    Mouse
  • Specificity

    This antibody inhibits T cell proliferation induced by dendritic cells.

  • Tested applications

    Suitable for: Flow Cyt, IHC-Fr, IP, Functional Studies, Inhibition Assay, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to CD70.

  • Positive control

    • IHC-Fr: Human tonsil tissue. Flow Cyt: Raji cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab77868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

IHC-Fr Use a concentration of 5 mg/ml.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
IHC-P 1/100. PubMed: 23776334
ELISA Use at an assay dependent concentration.

Target

  • Function

    Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 27L antibody
    • CD 70 antibody
    • CD27 L antibody
    • CD27 LG antibody
    • CD27 ligand antibody
    • CD27-L antibody
    • CD27L antibody
    • CD27LG antibody
    • CD70 antibody
    • CD70 antigen antibody
    • CD70 molecule antibody
    • CD70_HUMAN antibody
    • Ki 24 antigen antibody
    • Ki24 antigen antibody
    • Surface antigen CD70 antibody
    • TNFSF 7 antibody
    • TNFSF7 antibody
    • Tumor necrosis factor (ligand) superfamily, member 7 antibody
    • Tumor necrosis factor ligand superfamily member 7 antibody
    see all

Images

  • Overlay histograms showing left Raji and right SHSY5Y cells stained with ab77868 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab77868) (1x106 in 100µl at 1 µg/ml) for 30 min on ice.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.

     

  • Immunohistochemical analysis of Human tonsil tissue, staining CD70 with ab77868.

    Tissue was fixed with acetone and blocked with 6% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA in PBS) for 1 hour at 25°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

  • ab77868 used in Flow Cytometry. Red histogram represents cells stained with secondary alone ab8517 at a 1/200 dilution, blue histogram - Formaldehyde fixed SK-RC-45 human renal carcinoma cells stained with ab78868 at a 1/100 dilution. FL1-H - green channel

    See Abreview

References

This product has been referenced in:

  • Li S  et al. HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma. FEBS J 285:2278-2291 (2018). Read more (PubMed: 29676849) »
  • Liu L  et al. Breast cancer stem cells characterized by CD70 expression preferentially metastasize to the lungs. Breast Cancer N/A:N/A (2018). Read more (PubMed: 29948958) »
See all 6 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 02 2012

Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Specification
PBMCs
Preparation
Cell harvesting/tissue preparation method: Ficoll
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
FSC/SSC lymphocytes and then FSC-A/FSC-H singlets

Abcam user community

Verified customer

Submitted Oct 01 2012

Answer

Thank you for your inquiry.

I am afraid we have no data or protocols for the functional testing of this antibody. We rely on data provided by scientistsand we have not done functional studies in house with this antibody.The generation of the antibody is described in Schlossman, S.F. et al. 1995 Leukocyte Typing V.

I am sorry that this information could not be more helpful. Please contact us with any other questions.

Read More
Application
ELISA
Sample
Human Recombinant protein (purified CD70 ECD)
Specification
purified CD70 ECD
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Type
Direct

Tatiana Karakasheva

Verified customer

Submitted Jun 14 2011

Application
Flow Cytometry
Sample
Human Cell (SK-RC-45, renal cell carcinoma, human)
Specification
SK-RC-45, renal cell carcinoma, human
Preparation
Cell harvesting/tissue preparation method: brief trypsinization
Sample buffer: PBS+0.5%BSA
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
general cell population

Tatiana Karakasheva

Verified customer

Submitted Jun 14 2011

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