Recombinant Anti-CD70 antibody [TAN1-6] (ab281908)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [TAN1-6] to CD70
- Suitable for: ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-CD70 antibody [TAN1-6]
See all CD70 primary antibodies -
Description
Rat monoclonal [TAN1-6] to CD70 -
Host species
Rat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD70. Raised by immunizing LOU rats with soluble recombinant CD70:Fc fusion protein
Database link: O55237 -
Positive control
- ICC/IF: RAW 264.7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
TAN1-6 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 21948 Mouse
- SwissProt: O55237 Mouse
- Unigene: 42228 Mouse
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Alternative names
- CD 27L antibody
- CD 70 antibody
- CD27 L antibody
see all
Images
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Immunofluorescence analysis of paraformaldehyde-fixed RAW 264.7 cells labeling CD70 with the chimeric rabbit version of this antibody at 10 µg/ml for 1 hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom chimeric antibody, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab281908 has not yet been referenced specifically in any publications.