Overview

  • Product name

    Anti-CD73 antibody [10f1]
    See all CD73 primary antibodies
  • Description

    Mouse monoclonal [10f1] to CD73
  • Host species

    Mouse
  • Specificity

    This antibody reacts specifically with human 5'-nucleotidase cN-II (63kDa).
  • Tested applications

    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CD73.

Properties

Applications

Our Abpromise guarantee covers the use of ab59462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 63 kDa.
ICC 1/200 - 1/1000.
ICC/IF Use at an assay dependent concentration. PubMed: 20601304

Target

  • Function

    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease

    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities

    Belongs to the 5'-nucleotidase family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

References

This product has been referenced in:

  • Ceusters J  et al. From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species. Sci Rep 7:696 (2017). Read more (PubMed: 28386120) »
  • Kopher RA  et al. Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells. Bone 47:718-28 (2010). ICC/IF ; Human . Read more (PubMed: 20601304) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Horse Cell (Mesenchymal stem cells from umbililical cord blood)
Specification
Mesenchymal stem cells from umbililical cord blood
Preparation
Cell harvesting/tissue preparation method: Percoll gradient isolation from umbilical cord blood followed by selective cultivation
Sample buffer: DMEM +1% BSA
Permeabilization
No
Gating Strategy
based on FS/SS pattern , mesenchymal stem cells without debris

Mrs. Kristel Demeyere

Verified customer

Submitted Oct 22 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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