Overview

  • Product name

    Anti-CD73 antibody [7G2]
    See all CD73 primary antibodies
  • Description

    Mouse monoclonal [7G2] to CD73
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, IHC-Fr, Flow Cyt, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length purified Human placental CD73.

  • Positive control

    • Flow Cyt: Human peripheral blood lymphocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab54217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. Use 10µg per test.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
    • Involvement in disease

      Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
    • Sequence similarities

      Belongs to the 5'-nucleotidase family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    Images

    • ab54217 staining human peripheral blood lymphocytes by FACS analysis.
    • ab54217 staining cultured human bone marrow stem cells by ICC/IF. Cells were formaldehyde fixed, permeabilization not done prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with sample for 12 hours at 4°C. FITC conjugated rabbit polyclonal to mouse diluted 1/1000 was used as the secondary.

      See Abreview

    References

    This product has been referenced in:

    • Wang X  et al. Inhibition of overactive TGF-ß attenuates progression of heterotopic ossification in mice. Nat Commun 9:551 (2018). Read more (PubMed: 29416028) »
    • Landa-Solís C  et al. Behavior of multipotent stem cells isolated in mobilized peripheral blood from sheep after culture with human chondrogenic medium. Tissue Cell 52:116-123 (2018). ICC/IF ; Sheep . Read more (PubMed: 29857820) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-10 of 14 Abreviews or Q&A

    Answer

    Merci de nous avoir contactés.

    Malheureusement, nous n'avons pas d’hybridome dans notre catalogue.

    Je vous recommande de regarder sur le site Web Biocompare qui vous permettra de rechercher des d'anticorps et leurs fournisseurs. Le lien est le suivant:
    http://www.biocompare.com

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Answer

    Thank you for contacting us.

    We apologize for this error. The concentration should be read as 1-5ug/ml; the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you for your reply.

    I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.

    To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Question
    Answer

    Thank you for contacting us.

    Apologies for the delay in getting back to you. Unfortunately we do not have another batch of this product available. As I mentioned previously I would be happy to refund the item or issue a free unit of another product.

    Apologies for any inconvenience caused.

    Read More

    Answer

    I am sorry for the delay in responding to your request for another batch of ab54217.

    I have been in contact with the lab and unfortunately there is not another lot available. I would be happy to refund the item or issue a free unit of another product. Please do let me know how you would like to proceed.

    Looking forward to your reply.

    Read More

    Question
    Answer

    Thank you for responding to our survey.
    I am sorry to hear the replacement vial did not work as expected. I am currently in contact with the lab to identify whether another batch is available. I will be back in touch as soon as I can. If there is anything I can do in the meantime, please just let me know.
    Thank you for your patience.

    Read More

    Answer

    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    I am very pleased to hear you would like to accept our offer and test ab11415 and ab11155in [Sheep]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Sheep] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Adipose and bone marrow stem cells)
    Specification
    Adipose and bone marrow stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - TRITON x100 0,1%
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

    Ms. Vega Villar

    Verified customer

    Submitted Mar 29 2012

    Question
    Answer

    Merci de nous avoir contactés et désolé pour le délai de ma réponse.
    Je viens d'être informé que l'anti-CD73 clone [7G2] (référence ab54217) a été décrit pour des tests d'inhibition dans la publication suivante : Thomson LF, et al. Tissue Antigens 35(1):9-19, 1990. Je n'ai malheureusement pas accès à cet article et ne peut donc pas vous donner plus de détails sur le protocole utilisé.
    Nous ne fournissons pas cette référence sans conservateur dans le tampon.
    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Answer

    Thank you for your reply.

    For dilution of the antibodies ready to use in the experiment, as discussed in the previous email I can suggest to dilute antibodiesin PBS orTBS. It is sometimes helpful to include 0.2% Tween 20, however this may need to be optimized individually. If there is high background staining, then 2 to 5% blocking agent can also be included.

    You may like to consider reviewing our online protocols from our Scientific support protocols and troubleshooting pages. IHC documents are under Immunostaining. These will contain some further information that I hope may be helpful:

    https://www.abcam.com/index.html?pageconfig=popular_protocols

    Should you have any further questions, please do not hesitate to contact us.

    Read More

    1-10 of 14 Abreviews or Q&A

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