• Product name
    Anti-CD73 antibody [7G2]
    See all CD73 primary antibodies
  • Description
    Mouse monoclonal [7G2] to CD73
  • Host species
  • Tested applications
    Suitable for: IP, IHC-Fr, Flow Cyt, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length purified Human placental CD73.

  • Positive control
    • Flow Cyt: Human peripheral blood lymphocytes.



Our Abpromise guarantee covers the use of ab54217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. Use 10µg per test.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
    • Involvement in disease
      Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
    • Sequence similarities
      Belongs to the 5'-nucleotidase family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all


    • ab54217 staining human peripheral blood lymphocytes by FACS analysis.
    • ab54217 staining cultured human bone marrow stem cells by ICC/IF. Cells were formaldehyde fixed, permeabilization not done prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with sample for 12 hours at 4°C. FITC conjugated rabbit polyclonal to mouse diluted 1/1000 was used as the secondary.


    This product has been referenced in:
    • Wang X  et al. Inhibition of overactive TGF-ß attenuates progression of heterotopic ossification in mice. Nat Commun 9:551 (2018). Read more (PubMed: 29416028) »
    • Landa-Solís C  et al. Behavior of multipotent stem cells isolated in mobilized peripheral blood from sheep after culture with human chondrogenic medium. Tissue Cell 52:116-123 (2018). ICC/IF ; Sheep . Read more (PubMed: 29857820) »

    See all 15 Publications for this product

    Customer reviews and Q&As

    Merci de nous avoir contactés.

    Malheureusement, nous n'avons pas d’hybridome dans notre catalogue.

    Je vous recommande de regarder sur le site Web Biocompare qui vous permettra de rechercher des d'anticorps et leurs fourni...

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    Thank you for contacting us.

    We apologize for this error. The concentration should be read as 1-5ug/ml; the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

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    Thank you for your reply.

    I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
    (1) Redeemed against the original invoice if this hasn't already been paid.

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    Thank you for contacting us.

    Apologies for the delay in getting back to you. Unfortunately we do not have another batch of this product available. As I mentioned previously I would be happy to refund the item or issue a free unit of another ...

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    I am sorry for the delay in responding to your request for another batch of ab54217.

    I have been in contact with the lab and unfortunately there is not another lot available. I would be happy to refund the item or issue a free unit of anothe...

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    Thank you for responding to our survey.
    I am sorry to hear the replacement vial did not work as expected. I am currently in contact with the lab to identify whether another batch is available. I will be back in touch as soon as I can. If there is ...

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    Expiration date: 18-08-2012

    Expiration date: 18-08-2012

    I am very pleased to hear you would like to accept our offer and test ab11415 and ab11155in [Sheep]. Th...

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    Immunocytochemistry/ Immunofluorescence
    Horse Cell (Adipose and bone marrow stem cells)
    Adipose and bone marrow stem cells
    Yes - TRITON x100 0,1%
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

    Ms. Vega Villar

    Verified customer

    Submitted Mar 29 2012

    Merci de nous avoir contactés et désolé pour le délai de ma réponse.
    Je viens d'être informé que l'anti-CD73 clone [7G2] (référence ab54217) a été décrit pour des tests d'inhibition dans la publication suivante : Thomson LF, et al. Tissue Antigens...

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    Thank you for your reply.

    For dilution of the antibodies ready to use in the experiment, as discussed in the previous email I can suggest to dilute antibodiesin PBS orTBS. It is sometimes helpful to include 0.2% Tween 20, however this may ne...

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    1-10 of 14 Abreviews or Q&A


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