Anti-CD73 antibody (ab137595)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CD73 antibody
See all CD73 primary antibodies -
Description
Rabbit polyclonal to CD73 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 211-538 of Human CD73.
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Positive control
- H1299 whole cell lysate; MDAMB231 xenograft; HeLa and Raji cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 63 kDa. | |
| IHC-P | 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
Involvement in disease
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
Sequence similarities
Belongs to the 5'-nucleotidase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 281363 Cow
- Entrez Gene: 4907 Human
- Entrez Gene: 23959 Mouse
- Entrez Gene: 58813 Rat
- Omim: 129190 Human
- SwissProt: Q05927 Cow
- SwissProt: P21589 Human
- SwissProt: Q61503 Mouse
see all -
Alternative names
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
Images
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Western blot - Anti-CD73 antibody (ab137595)Anti-CD73 antibody (ab137595) at 1/1000 dilution + H1229 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody (ab137595)Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft labelling CD73 with ab137595 at 1/100 dilution.
References
This product has been referenced in:
- James EL et al. Replicatively senescent human fibroblasts reveal a distinct intracellular metabolic profile with alterations in NAD+ and nicotinamide metabolism. Sci Rep 6:38489 (2016). Read more (PubMed: 27924925) »
