Overview

  • Product name
  • Description
    Rabbit polyclonal to CD73
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human CD73.
    Database link: P21589

  • Positive control
    • Jurkat cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab175396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease
    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities
    Belongs to the 5'-nucleotidase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Images

  • Western blot analysis of extracts of various cell lines, using NT5E antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling CD73 with ab175396 at 1/100. Magnification: 400x.
  • Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175396. Blue DAPI for nuclear staining.
  • ab175396 staining CD73 in Rat olfactory stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) for 2 hours. An Alexa Fluor® 594-conjugated Goat polyclonal was used as the secondary antibody (1/500).

    See Abreview

References

This product has been referenced in:
  • Wang H  et al. The human umbilical cord stem cells improve the viability of OA degenerated chondrocytes. Mol Med Rep 17:4474-4482 (2018). Read more (PubMed: 29328479) »
  • Zhang L  et al. Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro. Oxid Med Cell Longev 2018:1023025 (2018). Read more (PubMed: 29599892) »

See all 9 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Monkey Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 28 2017

Application
Flow Cytometry
Sample
Mouse lemur Cell (Olfactory stem cells)
Permeabilization
Yes - Cold Methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 28 2017

Application
Flow Cytometry
Sample
Rat Cell (Olfactory stem cells)
Permeabilization
Yes - Methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (gingiva)
Antigen retrieval step
None
Permeabilization
No
Specification
gingiva
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 15 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
gelatina as blocking agent for 15 minute(s) · Concentration: 0.2% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Miss. Marina Colella

Verified customer

Submitted Sep 22 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cell)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Sep 15 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory stem cell)
Permeabilization
Yes - 0.1% Triton X100
Specification
Olfactory stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Aug 23 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory stem cell)
Permeabilization
Yes - 0.1% Triton X100
Specification
Olfactory stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Aug 23 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Cytrate pH6
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

Submitted Aug 11 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (colon and jejunum)
Antigen retrieval step
Heat mediated
Permeabilization
Yes - 0.1% Triton-X-100
Specification
colon and jejunum
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Z-FIX
Username

Abcam user community

Verified customer

Submitted Sep 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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