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Cardiovascular Hypoxia Associated Proteins
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Recombinant

Recombinant Anti-CD73 antibody [AD2] (ab130451)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)
  • Anti-CD73 antibody [AD2] (ab130451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AD2] to CD73
  • Suitable for: Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD73 antibody [AD2]
    See all CD73 primary antibodies
  • Description

    Mouse monoclonal [AD2] to CD73
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Flow Cyt: Human PBMCs.
  • General notes

    This product has switched from a hybridoma to recombinant production method on 21st September 2020.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.01% Sodium azide
    Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    AD2
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Hypoxia
    • Associated Proteins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Associated products

  • Alternative Versions

    • APC Anti-CD73 antibody [AD2], prediluted (ab155378)
    • PE Anti-CD73 antibody [AD2] (ab157335)
    • FITC Anti-CD73 antibody [AD2] (ab239246)
    • Anti-CD73 antibody [AD2] - BSA and Azide free (ab239603)
    • Alexa Fluor® 647 Anti-CD73 antibody [AD2] (ab243083)
    • Alexa Fluor® 488 Anti-CD73 antibody [AD2] (ab252554)
    • PE/Cy7® Anti-CD73 antibody [AD2] (ab253253)
    • PE/Cy5® Anti-CD73 antibody [AD2] (ab253267)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant mouse CD73 protein (His tag) (ab223023)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab130451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
Application Abreviews Notes
Flow Cyt (1)
1/500.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
Flow Cyt
1/500.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease

    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities

    Belongs to the 5'-nucleotidase family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P21589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4907 Human
    • Omim: 129190 Human
    • SwissProt: P21589 Human
    • Unigene: 153952 Human
    • Alternative names

      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    Images

    • Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)
      Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)

      Flow cytometric analysis of human PBMC cells labeling CD73 with ab130451 at 1/500 dilution (Right) compared with an isotype control (Left).

      Cells were blocked with 20 μg/mL human IgG for 15 minutes on ice. Primary antibody incubation for 30 minutes on ice.

      Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113) was used as secondary antibody for 30 minutes on ice in the dark. Cells were also stained with Anti-Mouse CD19 conjugated to AlexaFluor® 647 for 30 minutes on ice in the dark. Added 7-AAD in 1X PBS and incubated for 5 minutes.

    • Anti-CD73 antibody [AD2] (ab130451)
      Anti-CD73 antibody [AD2] (ab130451)

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab130451? Please let us know so that we can cite the reference in this datasheet.

    ab130451 has been referenced in 1 publication.

    • Borrione P  et al. CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression. J Immunol 162:6238-46 (1999). PubMed: 10229870

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Anti-CD73 antibody [AD2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (HEK-293T)
    Permeabilization
    No
    Gating Strategy
    Live cells
    Specification
    HEK-293T
    Read More

    Abcam user community

    Verified customer

    Submitted May 27 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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