Recombinant Anti-CD73 antibody [AD2] (ab130451)
![Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)](https://www.abcam.com/ps/products/130/ab130451/Images/ab130451-412385-anti-cd73-antibody-ad2-flow-cytometry-pbmc-human1.JPG "Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AD2] to CD73
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD73 antibody [AD2]
See all CD73 primary antibodies -
Description
Mouse monoclonal [AD2] to CD73 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Human PBMCs.
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General notes
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AD2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- APC Anti-CD73 antibody [AD2], prediluted (ab155378)
- PE Anti-CD73 antibody [AD2] (ab157335)
- FITC Anti-CD73 antibody [AD2] (ab239246)
- Anti-CD73 antibody [AD2] - BSA and Azide free (ab239603)
- Alexa Fluor® 647 Anti-CD73 antibody [AD2] (ab243083)
- Alexa Fluor® 488 Anti-CD73 antibody [AD2] (ab252554)
- PE/Cy7® Anti-CD73 antibody [AD2] (ab253253)
- PE/Cy5® Anti-CD73 antibody [AD2] (ab253267)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
1/500.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
1/500. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
Involvement in disease
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
Sequence similarities
Belongs to the 5'-nucleotidase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4907 Human
- Omim: 129190 Human
- SwissProt: P21589 Human
- Unigene: 153952 Human
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Alternative names
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
Images
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Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)
Flow cytometric analysis of human PBMC cells labeling CD73 with ab130451 at 1/500 dilution (Right) compared with an isotype control (Left).
Cells were blocked with 20 μg/mL human IgG for 15 minutes on ice. Primary antibody incubation for 30 minutes on ice.
Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113) was used as secondary antibody for 30 minutes on ice in the dark. Cells were also stained with Anti-Mouse CD19 conjugated to AlexaFluor® 647 for 30 minutes on ice in the dark. Added 7-AAD in 1X PBS and incubated for 5 minutes.
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![Anti-CD73 antibody [AD2] (ab130451)](https://www.abcam.com/ps/products/130/ab130451/Images/ab130451-3-benefits-of-recombinant-antibodies.png)
Anti-CD73 antibody [AD2] (ab130451)
References (1)
ab130451 has been referenced in 1 publication.
- Borrione P et al. CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression. J Immunol 162:6238-46 (1999). PubMed: 10229870
