Overview

  • Product name
    Anti-CD73 antibody [AD2] (Phycoerythrin)
    See all CD73 primary antibodies
  • Description
    Mouse monoclonal [AD2] to CD73 (Phycoerythrin)
  • Host species
    Mouse
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab157335 recognizes CD73 expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Human blood cells

Properties

Applications

Our Abpromise guarantee covers the use of ab157335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl per 100µl whole blood. The content of a vial (1 ml) is sufficient for 100 tests.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease
    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities
    Belongs to the 5'-nucleotidase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Images

  • Surface staining (flow cytometry) of human peripheral blood with anti-human CD73 PE (ab157335). 

References

ab157335 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
No
Sample
Horse Cell (whole blood, bone marrow derived mesenchymal stem)
Specification
whole blood, bone marrow derived mesenchymal stem
Gating Strategy
B and T lymphocytes in whole blood. Live cells in BM-MSCs
Preparation
Cell harvesting/tissue preparation method: Jugular venous blood was collected into Vacutainer Tubes. Bone Marrow was aspirated from sternum and BM-MSCs were isolated and cultured. After, BM-MSCs were detached by treating with tryspin and washed with PBS
Sample buffer: Whole blood was collected in EDTA and red cells were lysed with BD FACS Lysing Solution. BM-MSCs were resuspended in PBS

Abcam user community

Verified customer

Submitted Sep 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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