Recombinant
RabMAb

Recombinant Anti-CD73 antibody [EPR6114] - Low endotoxin, Azide free (ab227113)

Overview

  • Product name

    Anti-CD73 antibody [EPR6114] - Low endotoxin, Azide free
    See all CD73 primary antibodies
  • Description

    Rabbit monoclonal [EPR6114] to CD73 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to an internal sequence within amino acids 50-100 of Human CD73 (UniProt P21589).

  • Positive control

    • Human fetal liver tissue lysate; A375 and HepG2 cell lysates; Human tonsil tissue
  • General notes

    ab227113 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab227113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
    • Involvement in disease

      Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
    • Sequence similarities

      Belongs to the 5'-nucleotidase family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling CD73 with Purified ab133582 at 1:250 dilution (8.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133582).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling CD73 with Purified ab133582 at 1:2000 dilution (1.12 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133582).

    • This IHC data was generated using the same anti-CD73 antibody clone, EPR6114, in a different buffer formulation (cat# ab133582).

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD73 with ab133582 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Kawasaki T  et al. Six1 is required for mouse dental follicle cell and human periodontal ligament-derived cell proliferation. Dev Growth Differ : (2016). IF . Read more (PubMed: 27241908) »
    • Zhang HY  et al. Target tissue ectoenzyme CD39/CD73-expressing Foxp3+ regulatory T cells in patients with psoriasis. Clin Exp Dermatol 40:182-91 (2015). IHC ; Human . Read more (PubMed: 25284153) »
    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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