• Product name

    Anti-CD73 antibody [EPR6115]
    See all CD73 primary antibodies
  • Description

    Rabbit monoclonal [EPR6115] to CD73
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD73 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P21589

  • Positive control

    • WB: A375 cell lysate and human spleen and Human fetal liver tissue lysates. IHC-P: Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function

    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease

    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities

    Belongs to the 5'-nucleotidase family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD73 with unpurified ab124725 at a 1/100 dilution.

  • All lanes : Anti-CD73 antibody [EPR6115] (ab124725) at 1/2000 dilution (purified)

    Lane 1 : A375 cell lysate
    Lane 2 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-CD73 antibody [EPR6115] (ab124725) at 1/1000 dilution (unpurified)

    Lane 1 : A375 cell lysate
    Lane 2 : Human spleen tissue lystate
    Lane 3 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Lanes 1 & 3 : HRP-conjugated goat anti-rabbit IgG at 1/200 dilution
    Lane 2 : HRP-conjugate goat anti-rabbit IgG at 1/200 dilution

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD73 with purified ab124725 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD


This product has been referenced in:

  • L Ramos T  et al. MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry. Cell Commun Signal 14:2 (2016). WB ; Human . Read more (PubMed: 26754424) »
  • Geoghegan JC  et al. Inhibition of CD73 AMP hydrolysis by a therapeutic antibody with a dual, non-competitive mechanism of action. MAbs 8:454-67 (2016). WB ; Human . Read more (PubMed: 26854859) »
See all 3 Publications for this product

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