Anti-CD74 antibody [1B8] (ab223350)
![Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)](https://www.abcam.com/ps/products/223/ab223350/Images/ab223350-282665-anti-cd74-antibody-1b8-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)")
Key features and details
- Mouse monoclonal [1B8] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CD74 antibody [1B8]
See all CD74 primary antibodies -
Description
Mouse monoclonal [1B8] to CD74 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD74. Invariant chain. NP_001020329.1.
Database link: P04233 -
Positive control
- WB: Raji cell lysate. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1B8 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab223350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/1000. Predicted molecular weight: 33 kDa. |
Target
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Function
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. -
Sequence similarities
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 972 Human
- Omim: 142790 Human
- SwissProt: P04233 Human
- Unigene: 436568 Human
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Alternative names
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)
4% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223350 at a 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin (red) stain and DAPI (blue) nuclear stain.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223350 (D) Merge.
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![Western blot - Anti-CD74 antibody [1B8] (ab223350)](https://www.abcam.com/ps/products/223/ab223350/Images/ab223350-282666-anti-cd74-antibody-1b8-western-blot.jpg)
Western blot - Anti-CD74 antibody [1B8] (ab223350)Anti-CD74 antibody [1B8] (ab223350) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33-35 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesBlocking: 5% skimmed milk in TBST.
Protocols
References (0)
ab223350 has not yet been referenced specifically in any publications.
