For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cd74-antibody-1b8-ab223350.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity B Cells CD
Share by email

Anti-CD74 antibody [1B8] (ab223350)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)
  • Western blot - Anti-CD74 antibody [1B8] (ab223350)

Key features and details

  • Mouse monoclonal [1B8] to CD74
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG2a

You may also be interested in

Protein
Product image
Recombinant Human CD74 protein (ab156328)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-CD74 antibody [1B8]
    See all CD74 primary antibodies
  • Description

    Mouse monoclonal [1B8] to CD74
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD74. Invariant chain. NP_001020329.1.
    Database link: P04233

  • Positive control

    • WB: Raji cell lysate. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1B8
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human CD74 protein (ab156328)

Applications

Our Abpromise guarantee covers the use of ab223350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • Sequence similarities

    Contains 1 thyroglobulin type-1 domain.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Target information above from: UniProt accession P04233 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 972 Human
    • Omim: 142790 Human
    • SwissProt: P04233 Human
    • Unigene: 436568 Human
    • Alternative names

      • CD 74 antibody
      • CD74 antibody
      • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
      • CD74 antigen antibody
      • CD74 molecule antibody
      • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
      • CLIP antibody
      • DHLAG antibody
      • Gamma chain of class II antigens antibody
      • HG2A_HUMAN antibody
      • HLA class II histocompatibility antigen gamma chain antibody
      • HLA DR antigens associated invariant chain antibody
      • HLA DR gamma antibody
      • HLA-DR antigens-associated invariant chain antibody
      • HLA-DR-gamma antibody
      • HLADG antibody
      • HLADR antigens associated invariant chain antibody
      • Ia antigen associated invariant chain antibody
      • Ia antigen-associated invariant chain antibody
      • Ia associated invariant chain antibody
      • Ia gamma antibody
      • Ii antibody
      • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
      • la-gamma antibody
      • Major histocompatibility complex class II invariant chain antibody
      • MHC HLA DR gamma chain antibody
      • MHC HLA-DR gamma chain antibody
      • p33 antibody
      • p35 antibody
      • Protein 41 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)
      Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)

      4% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223350 at a 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin (red) stain and DAPI (blue) nuclear stain.

      (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223350 (D) Merge.

    • Western blot - Anti-CD74 antibody [1B8] (ab223350)
      Western blot - Anti-CD74 antibody [1B8] (ab223350)
      Anti-CD74 antibody [1B8] (ab223350) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg

      Secondary
      Goat Anti-Mouse IgG: HRP at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 33 kDa
      Observed band size: 33-35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 5 minutes


      Blocking: 5% skimmed milk in TBST.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab223350? Please let us know so that we can cite the reference in this datasheet.

    ab223350 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab223350.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.