Anti-CD74 antibody [1B8] (ab223350)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD74 antibody [1B8]
See all CD74 primary antibodies -
Description
Mouse monoclonal [1B8] to CD74 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD74. Invariant chain. NP_001020329.1.
Database link: P04233 -
Positive control
- WB: Raji cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1B8 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab223350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/1000. Predicted molecular weight: 33 kDa. |
Target
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Function
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. -
Sequence similarities
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 972 Human
- Omim: 142790 Human
- SwissProt: P04233 Human
- Unigene: 436568 Human
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Alternative names
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)](https://www.abcam.com/ps/products/223/ab223350/Images/ab223350-282665-anti-cd74-antibody-1b8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [1B8] (ab223350)4% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223350 at a 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin (red) stain and DAPI (blue) nuclear stain.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223350 (D) Merge.
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![Western blot - Anti-CD74 antibody [1B8] (ab223350)](https://www.abcam.com/ps/products/223/ab223350/Images/ab223350-282666-anti-cd74-antibody-1b8-western-blot.jpg)
Western blot - Anti-CD74 antibody [1B8] (ab223350)Anti-CD74 antibody [1B8] (ab223350) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33-35 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesBlocking: 5% skimmed milk in TBST.
References
ab223350 has not yet been referenced specifically in any publications.
