Key features and details
- Mouse monoclonal [1B8] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-CD74 antibody [1B8]
See all CD74 primary antibodies
DescriptionMouse monoclonal [1B8] to CD74
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human CD74. Invariant chain. NP_001020329.1.
Database link: P04233
- WB: Raji cell lysate. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab223350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 33 kDa.|
FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Sequence similaritiesContains 1 thyroglobulin type-1 domain.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
- Information by UniProt
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
4% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223350 at a 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin (red) stain and DAPI (blue) nuclear stain.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223350 (D) Merge.
Anti-CD74 antibody [1B8] (ab223350) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33-35 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Blocking: 5% skimmed milk in TBST.
ab223350 has not yet been referenced specifically in any publications.