Overview

  • Product name

    Anti-CD74 antibody [6D9]
    See all CD74 primary antibodies
  • Description

    Mouse monoclonal [6D9] to CD74
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD74 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P04233

  • Positive control

    • WB: Raji cell lysate. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    6D9
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Function

    Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • Sequence similarities

    Contains 1 thyroglobulin type-1 domain.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

Images

  • 4% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223357 at 1/100 dilution for 60 min at room temperature in ICC/IF, followed by Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 min at room temperature.

    (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223357 (D) Merge.

  • Anti-CD74 antibody [6D9] (ab223357) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa


    Exposure time: 5 minutes


    Blocking buffer: 5% skim milk in TBST

    Primary incubation: 2 hours at room temperature.

References

ab223357 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223357.
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