Key features and details
- Mouse monoclonal [6D9] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD74 antibody [6D9]
See all CD74 primary antibodies
DescriptionMouse monoclonal [6D9] to CD74
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD74 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P04233
- WB: Raji cell lysate. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab223357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Sequence similaritiesContains 1 thyroglobulin type-1 domain.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
- Information by UniProt
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
4% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223357 at 1/100 dilution for 60 min at room temperature in ICC/IF, followed by Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 min at room temperature.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223357 (D) Merge.
Anti-CD74 antibody [6D9] (ab223357) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Goat Anti-Mouse IgG HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 5 minutes
Blocking buffer: 5% skim milk in TBST
Primary incubation: 2 hours at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223357 has not yet been referenced specifically in any publications.