Anti-CD74 antibody (ab155784)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CD74 antibody
See all CD74 primary antibodies -
DescriptionRabbit polyclonal to CD74
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-296 of Human CD74 (P04233).
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Positive control
- Human colon cancer tissue; Raji whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79% PBS, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 34 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
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Sequence similaritiesContains 1 thyroglobulin type-1 domain.
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Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 972 Human
- Omim: 142790 Human
- SwissProt: P04233 Human
- Unigene: 436568 Human
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Alternative names
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
see all
Images
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Western blot - Anti-CD74 antibody (ab155784)All lanes : Anti-CD74 antibody (ab155784) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : K652 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody (ab155784)Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue, labeling CD74 with ab155784 at 1/500 dilution.
References
ab155784 has not yet been referenced specifically in any publications.
