Key features and details
- Mouse monoclonal [Bu45] to CD74
- Suitable for: ICC/IF, Functional Studies, IP, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD74 antibody [Bu45]
See all CD74 primary antibodies
DescriptionMouse monoclonal [Bu45] to CD74
Tested applicationsSuitable for: ICC/IF, Functional Studies, IP, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
B lymphoblastoid cell line HFB1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab54428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 10µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Sequence similaritiesContains 1 thyroglobulin type-1 domain.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
- Information by UniProt
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
ab54428 has not yet been referenced specifically in any publications.