Anti-CD74 antibody [EPR4064] (ab108393)


  • Product name
    Anti-CD74 antibody [EPR4064]
    See all CD74 primary antibodies
  • Description
    Rabbit monoclonal [EPR4064] to CD74
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD74 aa 1-100. The exact sequence is proprietary.
    Database link: P04233

  • Positive control
    • Ramos cell lysate; Human tonsil tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab108393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/150.
ICC/IF 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
    • Sequence similarities
      Contains 1 thyroglobulin type-1 domain.
    • Cellular localization
      Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 74 antibody
      • CD74 antibody
      • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
      • CD74 antigen antibody
      • CD74 molecule antibody
      • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
      • CLIP antibody
      • DHLAG antibody
      • Gamma chain of class II antigens antibody
      • HG2A_HUMAN antibody
      • HLA class II histocompatibility antigen gamma chain antibody
      • HLA DR antigens associated invariant chain antibody
      • HLA DR gamma antibody
      • HLA-DR antigens-associated invariant chain antibody
      • HLA-DR-gamma antibody
      • HLADG antibody
      • HLADR antigens associated invariant chain antibody
      • Ia antigen associated invariant chain antibody
      • Ia antigen-associated invariant chain antibody
      • Ia associated invariant chain antibody
      • Ia gamma antibody
      • Ii antibody
      • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
      • la-gamma antibody
      • Major histocompatibility complex class II invariant chain antibody
      • MHC HLA DR gamma chain antibody
      • MHC HLA-DR gamma chain antibody
      • p33 antibody
      • p35 antibody
      • Protein 41 antibody
      see all


    • Confocal image of ab108393 staining CD74 in the RAMOS (human Burkitt's lymphoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500) and an Alexa Fluor® 488-conjugated goat anti-rabbit  IgG polyclonal (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used ad a nuclear counterstain at 1/200.

    • ab108393 staining CD74 in RAMOS (human Burkitt's lymphoma) cell line. The sample was fixed with 4% paraformaldehyde and incubated with the primary antibody (1/150) for 30 minutes at 4°C. An Alexa Fluor® 488 -conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.

      Control: Cell without incubation with primary antibody and secondary antibody (Blue).

    • Anti-CD74 antibody [EPR4064] (ab108393) at 1/1000 dilution + Ramos cell lysate at 10 µg

      Predicted band size: 34 kDa

    • Immunohistochemical analysis of CD74 in paraffin embedded Human tonsil tissue, using ab108393 at a 1/100 dilution.
    • ab108393 showing negative staining in Normal brain tissue.

    • ab108393 showing positive staining in B cell lymphoma tissue.

    • ab108393 showing negative staining in Normal placenta tissue.

    • ab108393 showing positive staining in Normal spleen tissue.

    • ab108393 showing negative staining in Normal heart tissue.

    • ab108393 showing negative staining in Normal liver tissue.


    ab108393 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us.

    The immunogen used to generate ab108393 maps between amino acids 15 and 46 of human CD74 (Swiss Prot: P04233) which is within cytoplasmic domain.

    However, ...

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    Vielen Dank für Ihren Anruf gestern. Ich kann Ihnen nun bestätigen, dass ab108393 (der monoklonale Antikörper aus den Rabbit, über den wir gesprochen haben) ebenfalls mit Citrate Puffer (pH6) als Antigen-Demaskierungspuffer im Labor getestet wurde....

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