Recombinant Anti-CD74 antibody [EPR4064] - BSA and Azide free (ab247655)
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- References
- Protocols
Overview
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Product name
Anti-CD74 antibody [EPR4064] - BSA and Azide free
See all CD74 primary antibodies -
Description
Rabbit monoclonal [EPR4064] to CD74 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD74 aa 1-100. The exact sequence is proprietary.
Database link: P04233 -
General notes
ab247655 is the carrier-free version of ab108393 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247655 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4064 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 34 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. -
Sequence similarities
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 972 Human
- Omim: 142790 Human
- SwissProt: P04233 Human
- Unigene: 436568 Human
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Alternative names
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
see all
Datasheets and documents
References
ab247655 has not yet been referenced specifically in any publications.
