Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [In-1] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
Product nameAnti-CD74 antibody [In-1]
See all CD74 primary antibodies
DescriptionRabbit monoclonal [In-1] to CD74
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to CD74. Mouse lymphocytes of strain A injected into Wistar rats.
- ICC/IF: Mouse splenocytes. WB: Mouse spleen and lymph node lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Proclin 300
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab245692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.0001 µg/ml. Predicted molecular weight: 35 kDa.|
FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Sequence similaritiesContains 1 thyroglobulin type-1 domain.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
- Information by UniProt
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
All lanes : Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/ml (incubated for 1 hr)
Lane 1 : Mouse spleen lysate (RIPA buffer)
Lane 2 : Mouse lymph node lysate (RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE gel.
Detected by chemiluminescence.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245692 has not yet been referenced specifically in any publications.