Overview

  • Product name

    Anti-CD74 antibody [LN2]
    See all CD74 primary antibodies
  • Description

    Mouse monoclonal [LN2] to CD74
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    SU-DHL-4 lymphoma cells.

  • Positive control

    • Human tonsil normal tissue lysate - total protein: Hum an tonsil and Human liver tissues; Raji cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    LN2
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 5 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • Sequence similarities

    Contains 1 thyroglobulin type-1 domain.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

Images

  • Ab9514 staining CD74 in paraffin embedded Human tonsil tissue sections by Immunohistochemistry (IHC-P).

  • Anti-CD74 antibody [LN2] (ab9514) at 5 µg/ml + Human tonsil normal tissue lysate - total protein (ab29615) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa
    Additional bands at: 37 kDa (possible post-translational modification)

  • Ab9514 staining CD74 in paraffin embedded Human tonsil tissue sections by Immunohistochemistry (IHC-P).
  • ab9514 staining CD74 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/75 dilution for 30 minutes at 20°C. A HRP-conjugated rat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.

    See Abreview

  • Overlay histogram showing Raji cells stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9514, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:

  • Ha W  et al. Ibudilast sensitizes glioblastoma to temozolomide by targeting Macrophage Migration Inhibitory Factor (MIF). Sci Rep 9:2905 (2019). Read more (PubMed: 30814573) »
  • De R  et al. Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF-?B signaling. J Biol Chem 293:19740-19760 (2018). Read more (PubMed: 30366984) »
See all 14 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Neutral Buffered Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 29 2010

Answer

You could use any unrelated antibody for a negative control, we don't have a specific one to recommend. You can also run a negative control using the same conditions that would be appropriate for antibody staining, replacing the primary antibody with blocking serum. Ab9514 is provided in the form of tissue culture supernatant, and since this is unpurified material, we are unable to provide an exact concentration.

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Question
Answer

Thank you for your enquiry. There is no particular negative control tissue for CD74; the negative control should be run on the same tissue (known positive) but without primary antibody. Please contact us again if you have any additional questions.

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