Key features and details
- Mouse monoclonal [B-L5] to CD75
- Suitable for: Flow Cyt, IP
- Reacts with: Human
- Isotype: IgM
Product nameAnti-CD75 antibody [B-L5]
See all CD75 primary antibodies
DescriptionMouse monoclonal [B-L5] to CD75
Tested applicationsSuitable for: Flow Cyt, IPmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD75. Raji cell line.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 98.91% PBS, 1% BSA
Concentration information loading...
Purification notesThis antibody was purified by gel filtration.
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTransfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 29 family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
Cellular localizationGolgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
- Information by UniProt
- 6 sialyltransferase antibody
- 6-sialyltransferase 1 antibody
- 6-ST 1 antibody
CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 47kDa; CD75
Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.
ab27316 has not yet been referenced specifically in any publications.