• Product name

    Anti-CD75 antibody [B-L5]
    See all CD75 primary antibodies
  • Description

    Mouse monoclonal [B-L5] to CD75
  • Host species

  • Specificity

    This antibody recognises CD75.
  • Tested applications

    Suitable for: Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Raji cell line.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purification notes

    This antibody was purified by gel filtration.
  • Clonality

  • Clone number

  • Myeloma

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab27316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10 µl to label 106 cells or 100 µl of whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
    • Pathway

      Protein modification; protein glycosylation.
    • Sequence similarities

      Belongs to the glycosyltransferase 29 family.
    • Post-translational

      The soluble form derives from the membrane form by proteolytic processing.
      The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
    • Cellular localization

      Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
    • Information by UniProt
    • Database links

    • Alternative names

      • 6 sialyltransferase antibody
      • 6-sialyltransferase 1 antibody
      • 6-ST 1 antibody
      • Alpha 2 antibody
      • Alpha 2,6 ST 1 antibody
      • Alpha 2,6 ST antibody
      • B cell antigen CD75 antibody
      • B-cell antigen CD75 antibody
      • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
      • Beta-galactoside alpha-2 antibody
      • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
      • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
      • MGC48859 antibody
      • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
      • Sialyltransferase 1 antibody
      • SIAT1 antibody
      • SIAT1_HUMAN antibody
      • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
      • ST6Gal I antibody
      • ST6GAL1 antibody
      • ST6GalI antibody
      • ST6N antibody
      see all


    • CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.

      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

      Band: 47kDa; CD75

    • Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.


    ab27316 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Thank you for contacting us. ab99597 is an allophycocyanin conjugated secondary antibody specific to primary Mouse IgM, it would therefore recognise ab27316 (a Mouse IgM). It would not recognise IgG. For more details about secondary antibodies please visit www.abcam.com/secondary and https://www.abcam.com/index.html?pageconfig=productmap&cl=918. About isotype controls, we have a guide that can be found at https://www.abcam.com/index.html?pageconfig=technicalfaqs#5 For ab53318 (mouse monoclonal IgG2a - FITC) we have ab81197 For ab81289 (rabbit monoclonal IgG) we have ab119749 For ab19622 (rat monoclonal IgG2b - FITC) we have ab37364 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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