Product nameAnti-CD75 antibody [EPR22054-238]
See all CD75 primary antibodies
DescriptionRabbit monoclonal [EPR22054-238] to CD75
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CD75 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P15907
- WB: Daudi, Raji and THP-1 whole cell lysates. ICC/IF: Daudi cells. Flow Cyt: Daudi cells. IP: Raji whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab225793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).|
FunctionTransfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 29 family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
Cellular localizationGolgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
- Information by UniProt
- 6 sialyltransferase antibody
- 6-sialyltransferase 1 antibody
- 6-ST 1 antibody
All lanes : Anti-CD75 antibody [EPR22054-238] (ab225793) at 1/1000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HFF-1 (human skin fibroblast cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with the literature (PMID 23358684).
Negative control: HFF-1 (PMID: 23358684).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (human Burkitt's lymphoma cell line) and HFF-1 (human skin fibroblast cell line) cells labeling CD75 with ab225793 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining in Daudi cells, likely localized to the Golgi (PMID: 23578204).
Negative control: HFF-1 (human skin fibroblast) [PMID: 23358684].
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HFF-1 (human skin fibroblast cell line; Left panel) and Daudi (human Burkitt's lymphoma cell line; Right panel) cell lines labeling CD75 with ab225793 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: HFF-1 [PMID: 23358684].
CD75 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab225793 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225793 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Raji whole cell lysate 10 µg (Input).
Lane 2: ab225793 IP in Raji whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225793 in Raji whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
ab225793 has not yet been referenced specifically in any publications.