Recombinant
RabMAb

Recombinant Anti-CD75 antibody [EPR22054-238] (ab225793)

Overview

  • Product name

    Anti-CD75 antibody [EPR22054-238]
    See all CD75 primary antibodies
  • Description

    Rabbit monoclonal [EPR22054-238] to CD75
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD75 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P15907

  • Positive control

    • WB: Daudi, Raji and THP-1 whole cell lysates. ICC/IF: Daudi cells. Flow Cyt: Daudi cells. IP: Raji whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22054-238
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.
ICC/IF 1/50.
IP 1/30.
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 29 family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6 sialyltransferase antibody
    • 6-sialyltransferase 1 antibody
    • 6-ST 1 antibody
    • Alpha 2 antibody
    • Alpha 2,6 ST 1 antibody
    • Alpha 2,6 ST antibody
    • B cell antigen CD75 antibody
    • B-cell antigen CD75 antibody
    • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
    • Beta-galactoside alpha-2 antibody
    • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
    • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
    • MGC48859 antibody
    • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
    • Sialyltransferase 1 antibody
    • SIAT1 antibody
    • SIAT1_HUMAN antibody
    • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
    • ST6Gal I antibody
    • ST6GAL1 antibody
    • ST6GalI antibody
    • ST6N antibody
    see all

Images

  • All lanes : Anti-CD75 antibody [EPR22054-238] (ab225793) at 1/1000 dilution

    Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : HFF-1 (human skin fibroblast cell line) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with the literature (PMID 23358684).

    Negative control: HFF-1 (PMID: 23358684).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (human Burkitt's lymphoma cell line) and HFF-1 (human skin fibroblast cell line) cells labeling CD75 with ab225793 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining in Daudi cells, likely localized to the Golgi (PMID: 23578204).

    Negative control: HFF-1 (human skin fibroblast) [PMID: 23358684].

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HFF-1 (human skin fibroblast cell line; Left panel) and Daudi (human Burkitt's lymphoma cell line; Right panel) cell lines labeling CD75 with ab225793 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HFF-1 [PMID: 23358684].

  • CD75 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab225793 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225793 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10 µg (Input). 

    Lane 2: ab225793 IP in Raji whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225793 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

References

ab225793 has not yet been referenced specifically in any publications.

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