Overview

  • Product name

    Anti-CD75 antibody [LN1]
    See all CD75 primary antibodies
  • Description

    Mouse monoclonal [LN1] to CD75
  • Host species

    Mouse
  • Specificity

    This antibody is shown to be a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation - Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

  • Positive control

    • Lymph node or human tonsil.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    LN1
  • Isotype

    IgM
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Flow Cyt Use 1µg for 106 cells.

ab18400 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 29 family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6 sialyltransferase antibody
    • 6-sialyltransferase 1 antibody
    • 6-ST 1 antibody
    • Alpha 2 antibody
    • Alpha 2,6 ST 1 antibody
    • Alpha 2,6 ST antibody
    • B cell antigen CD75 antibody
    • B-cell antigen CD75 antibody
    • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
    • Beta-galactoside alpha-2 antibody
    • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
    • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
    • MGC48859 antibody
    • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
    • Sialyltransferase 1 antibody
    • SIAT1 antibody
    • SIAT1_HUMAN antibody
    • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
    • ST6Gal I antibody
    • ST6GAL1 antibody
    • ST6GalI antibody
    • ST6N antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil stained with ab77676 at 4-8ug/ml, using a peroxidase-conjugate secondary and AEC chromogen. Note cell membrane and cytoplasmic staining of B cells in germinal center.
  • ICC/IF image of ab77676 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77676, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing Ramos cells stained with ab77676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77676, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Zhao Y  et al. a2,6-Sialylation mediates hepatocellular carcinoma growth in vitro and in vivo by targeting the Wnt/ß-catenin pathway. Oncogenesis 6:e343 (2017). WB . Read more (PubMed: 28553930) »
  • Collier AJ  et al. Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States. Cell Stem Cell 20:874-890.e7 (2017). Read more (PubMed: 28343983) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for your recent telephone call. I am sorry to confirm that the epitope for ab77676 CD75 antibody [LN1] has regrettably not been mapped. We aim to provide as much information as we can to customers, and I am sorry we do not have the data from our source on this occasion. Should you have any further questions, please do not hesitate to contact us.

Read More

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