Recombinant Anti-CD79a antibody [EP3618] KO Tested (ab79414)

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Western blot - Anti-CD79a antibody [EP3618] (ab79414)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3618] to CD79a
Suitable for: ICC/IF, WB, IHC-P
Knockout validated
Reacts with: Human

Carrier Free

Product name

Anti-CD79a antibody [EP3618]
See all CD79a primary antibodies
Description

Rabbit monoclonal [EP3618] to CD79a
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human CD79a aa 50-150 (extracellular). The exact sequence is proprietary.
Positive control
- Raji cell, Daudi cell, human tonsil tissue and human spleen tissue lysates. Raji cells. Human tonsil tissue. WB: Wild-type Raji cell lysate and Daudi cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.16 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP3618
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD79a antibody [EP3618] - BSA and Azide free (ab239891)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Raji membrane extract lysate (ab30126)
Recombinant Protein
- Recombinant Human CD79a protein (His tag) (ab227390)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/500.
WB		1/5000 - 1/20000. Detects a band of approximately 44-48 kDa (predicted molecular weight: 25 kDa).
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Notes
ICC/IF 1/500.
WB 1/5000 - 1/20000. Detects a band of approximately 44-48 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP.

Function

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
Tissue specificity

B-cells.
Involvement in disease

Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 ITAM domain.
Post-translational
modifications

Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
Cellular localization

Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
Target information above from: UniProt accession P11912 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 973 Human
- Omim: 112205 Human
- SwissProt: P11912 Human
- Unigene: 631567 Human
Alternative names
- B lymphocyte-specific MB1 protein antibody
- B-cell antigen receptor complex-associated protein alpha chain antibody
- CD 79a antibody
- CD79a antibody
- CD79a antigen (immunoglobulin-associated alpha) antibody
- CD79A antigen antibody
- CD79a molecule, immunoglobulin-associated alpha antibody
- CD79A_HUMAN antibody
- Ig alpha antibody
- Ig-alpha antibody
- IGA antibody
- IgM-alpha antibody
- Immunoglobulin-associated alpha antibody
- Ly54 antibody
- MB-1 membrane glycoprotein antibody
- MB1 antibody
- Membrane-bound immunoglobulin-associated protein antibody
- Surface IgM-associated protein antibody
see all

Western blot - Anti-CD79a antibody [EP3618] (ab79414)

All lanes : Anti-CD79a antibody [EP3618] (ab79414) at 1/5000 dilution

Lane 1 : Wild-type Raji cell lysate
Lane 2 : CD79A knockout Raji cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HAP1 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 25 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-CD79a antibody [EP3618] staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab79414 was shown to bind specifically to CD79a. A band was observed at 40-50 kDa in wild-type Raji cell lysates with no signal observed at this size in CD79A knockout cell line ab274911 (knockout cell lysate ab281361). To generate this image, wild-type and CD79A knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 at 1/100 dilution staining CD79a in paraffin-embedded human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-CD79a antibody [EP3618] (ab79414)

All lanes : Anti-CD79a antibody [EP3618] (ab79414) at 1/10000 dilution

Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Human tonsil tissue lysate
Lane 4 : Human spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 44-48 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EP3618] (ab79414)

Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) cells labelling CD79a with purified ab79414 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal spleen tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal brain tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal colon tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal heart tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal brain tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal heart tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD79a antibody [EP3618] (ab79414)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CD79a antibody [EP3618] (ab79414)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab79414? Please let us know so that we can cite the reference in this datasheet.

ab79414 has been referenced in 4 publications.

Caetano AJ et al. Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease. Elife 10:N/A (2021). PubMed: 33393902
Wen S et al. Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney. Front Oncol 10:609839 (2020). PubMed: 33585230
Vanshylla K et al. The extracellular membrane-proximal domain of membrane-bound IgE restricts B cell activation by limiting B cell antigen receptor surface expression. Eur J Immunol 48:441-453 (2018). PubMed: 29150831
Yang X et al. Misdiagnosis of primary pleural DLBCL as tuberculosis: A case report and literature review. Mol Clin Oncol 8:729-732 (2018). PubMed: 29732155

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Using Anti-CD79a antibody [EP3618] (ab79414) with expected cell membrane staining in lymph tissues, but also seeing a lot of nuclear staining in colon and prostate tissues. Is this validated?

Abcam community

Verified customer

Asked on Mar 12 2012

Answer

According to our records, ab79414 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.

Abcam Scientific Support

Answered on Mar 12 2012

Question

Using Anti-CD79a antibody [EP3618] (ab79414) with expected cell membrane staining in lymph tissues, but also seeing a lot of nuclear staining in colon and prostate tissues. Is this validated?

Abcam community

Verified customer

Asked on Feb 23 2012

Answer

Thank you for contacting Abcam regarding ab79414.

The localization of this protein should be to the membrane. The only other information we have regarding this is that following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. We have not specifically tested colon and prostate tissues so I cannot say for sure that this is accurate staining.

If you would provide a summary of your protocol and some images, I would be happy to investigate this further. Additionally, examining the literature for the expected expression profile in your tissues of interest might provide more specific details.

I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

Abcam Scientific Support

Answered on Feb 23 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (4)

Customer reviews and Q&As

Dissociation constant (K_D)

Post-translational
modifications