Recombinant
RabMAb

Recombinant Anti-CD79a antibody [EP3618] (ab79414)

Overview

  • Product name

    Anti-CD79a antibody [EP3618]
    See all CD79a primary antibodies
  • Description

    Rabbit monoclonal [EP3618] to CD79a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD79a aa 50-150 (extracellular). The exact sequence is proprietary.

  • Positive control

    • Raji cell, Daudi cell, human tonsil tissue and human spleen tissue lysates. Raji cells. Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab79414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/5000 - 1/20000. Detects a band of approximately 44-48 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
    • Tissue specificity

      B-cells.
    • Involvement in disease

      Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
    • Cellular localization

      Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
    • Information by UniProt
    • Database links

    • Alternative names

      • B lymphocyte-specific MB1 protein antibody
      • B-cell antigen receptor complex-associated protein alpha chain antibody
      • CD 79a antibody
      • CD79a antibody
      • CD79a antigen (immunoglobulin-associated alpha) antibody
      • CD79A antigen antibody
      • CD79a molecule, immunoglobulin-associated alpha antibody
      • CD79A_HUMAN antibody
      • Ig alpha antibody
      • Ig-alpha antibody
      • IGA antibody
      • IgM-alpha antibody
      • Immunoglobulin-associated alpha antibody
      • Ly54 antibody
      • MB-1 membrane glycoprotein antibody
      • MB1 antibody
      • Membrane-bound immunoglobulin-associated protein antibody
      • Surface IgM-associated protein antibody
      see all

    Images

    • ab79414 at 1/100 dilution staining CD79a in paraffin-embedded human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-CD79a antibody [EP3618] (ab79414) at 1/10000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Human tonsil tissue lysate
      Lane 4 : Human spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 25 kDa
      Observed band size: 44-48 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) cells labelling CD79a with purified ab79414 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Flow cytometric analysis of Raji cells using ab79414 at a 1/140 dilution (red) or a rabbit IgG (negative) (green).
    • ab79414 showing positive staining in Normal spleen tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing negative staining in Normal brain tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing negative staining in Normal colon tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing negative staining in Normal heart tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing negative staining in Normal brain tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

       

    • ab79414 showing negative staining in Normal colon tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing negative staining in Normal heart tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab79414 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab79414 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    According to our records, ab79414 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

    If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

    We wish you the best of luck with your research and look forward to a reply.

    Read More

    Answer

    Thank you for contacting Abcam regarding ab79414.


    The localization of this protein should be to the membrane. The only other information we have regarding this is that following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. We have not specifically tested colon and prostate tissues so I cannot say for sure that this is accurate staining.


    If you would provide a summary of your protocol and some images, I would be happy to investigate this further. Additionally, examining the literature for the expected expression profile in your tissues of interest might provide more specific details.


    I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

    Read More

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