Recombinant
RabMAb

Recombinant Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)

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Overview

  • Product name

    Anti-CD79a antibody [EPR3619] - BSA and Azide free
    See all CD79a primary antibodies

  • Description

    Rabbit monoclonal [EPR3619] to CD79a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD79a aa 150-250. The exact sequence is proprietary.
    Database link: P11912

  • General notes

    ab248518 is the carrier-free version of ab133483 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248518 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab248518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.

    • Tissue specificity

      B-cells.

    • Involvement in disease

      Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 ITAM domain.

    • Post-translational
      modifications

      Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

    • Cellular localization

      Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
    • Information by UniProt

    • Database links

      • Alternative names

        • B lymphocyte-specific MB1 protein antibody
        • B-cell antigen receptor complex-associated protein alpha chain antibody
        • CD 79a antibody
        • CD79a antibody
        • CD79a antigen (immunoglobulin-associated alpha) antibody
        • CD79A antigen antibody
        • CD79a molecule, immunoglobulin-associated alpha antibody
        • CD79A_HUMAN antibody
        • Ig alpha antibody
        • Ig-alpha antibody
        • IGA antibody
        • IgM-alpha antibody
        • Immunoglobulin-associated alpha antibody
        • Ly54 antibody
        • MB-1 membrane glycoprotein antibody
        • MB1 antibody
        • Membrane-bound immunoglobulin-associated protein antibody
        • Surface IgM-associated protein antibody
        see all

      References

      ab248518 has not yet been referenced specifically in any publications.

      Publishing research using ab248518? Please let us know so that we can cite the reference in this datasheet.

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