RecombinantRabMAb

Recombinant Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)

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Western blot - Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)
  • Flow Cytometry - Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)
  • Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3619] to CD79a - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD79a antibody [EPR3619] - BSA and Azide free
    See all CD79a primary antibodies

  • Description

    Rabbit monoclonal [EPR3619] to CD79a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD79a aa 150-250. The exact sequence is proprietary.
    Database link: P11912

  • General notes

    ab248518 is the carrier-free version of ab133483 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248518 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab248518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.

    • Tissue specificity

      B-cells.

    • Involvement in disease

      Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 ITAM domain.

    • Post-translational
      modifications

      Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

    • Cellular localization

      Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
    • Information by UniProt

    • Database links

      • Alternative names

        • B lymphocyte-specific MB1 protein antibody
        • B-cell antigen receptor complex-associated protein alpha chain antibody
        • CD 79a antibody
        • CD79a antibody
        • CD79a antigen (immunoglobulin-associated alpha) antibody
        • CD79A antigen antibody
        • CD79a molecule, immunoglobulin-associated alpha antibody
        • CD79A_HUMAN antibody
        • Ig alpha antibody
        • Ig-alpha antibody
        • IGA antibody
        • IgM-alpha antibody
        • Immunoglobulin-associated alpha antibody
        • Ly54 antibody
        • MB-1 membrane glycoprotein antibody
        • MB1 antibody
        • Membrane-bound immunoglobulin-associated protein antibody
        • Surface IgM-associated protein antibody
        see all

      Images

      • Western blot - Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)
        Western blot - Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)
        All lanes : Anti-CD79a antibody [EPR3619] (ab133483) at 1/1000 dilution

        Lane 1 : Raji cell lysate
        Lane 2 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 25 kDa



        This data was developed using ab133483, the same antibody clone in a different buffer formulation.

      • Flow Cytometry - Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)
        Flow Cytometry - Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)
        This data was developed using ab133483, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilised Ramos cells labelled with ab133483 at 1/100 dilution (red) or negative rabbit IgG (green).
      • Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)
        Anti-CD79a antibody [EPR3619] - BSA and Azide free (ab248518)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248518? Please let us know so that we can cite the reference in this datasheet.

      ab248518 has not yet been referenced specifically in any publications.

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