Overview

  • Product name

    Anti-CD79a antibody [JCB117 + HM47A9]
    See all CD79a primary antibodies
  • Description

    Mouse monoclonal [JCB117 + HM47A9] to CD79a
  • Host species

    Mouse
  • Specificity

    ab199001 is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with anti-CD20. ab199001 will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human CD79a.
    Sequence:

    GTYQDVGSLNIADVQ

  • Positive control

    • Daudi or Ramos cells. Germinal center B-cells in a lymph node or tonsil.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    Both antibodies (JCB117 + HM47/A9) are mouse IgG1 kappa.

Properties

Applications

Our Abpromise guarantee covers the use of ab199001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 25 kDa).
IP Use at 1-0.5 µg/mg of lysate.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
  • Tissue specificity

    B-cells.
  • Involvement in disease

    Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
  • Cellular localization

    Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • B lymphocyte-specific MB1 protein antibody
    • B-cell antigen receptor complex-associated protein alpha chain antibody
    • CD 79a antibody
    • CD79a antibody
    • CD79a antigen (immunoglobulin-associated alpha) antibody
    • CD79A antigen antibody
    • CD79a molecule, immunoglobulin-associated alpha antibody
    • CD79A_HUMAN antibody
    • Ig alpha antibody
    • Ig-alpha antibody
    • IGA antibody
    • IgM-alpha antibody
    • Immunoglobulin-associated alpha antibody
    • Ly54 antibody
    • MB-1 membrane glycoprotein antibody
    • MB1 antibody
    • Membrane-bound immunoglobulin-associated protein antibody
    • Surface IgM-associated protein antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD79a with ab199001 at 1µg/mL.

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD79a with ab199001 at 1µg/mL.

References

This product has been referenced in:

  • Minguet S  et al. Caveolin-1-dependent nanoscale organization of the BCR regulates B cell tolerance. Nat Immunol 18:1150-1159 (2017). Read more (PubMed: 28805811) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Permeabilization
No
Specification
spleen
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 03 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Specification
lymph node
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Prof. 清和 尾崎

Verified customer

Submitted Sep 21 2016

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