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RabMAb

Anti-CD79a antibody [SP18], prediluted (ab27607)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18], prediluted (ab27607)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18], prediluted (ab27607)

Key features and details

  • Rabbit monoclonal [SP18] to CD79a, prediluted
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CD79a antibody [SP18], prediluted
    See all CD79a primary antibodies

  • Description

    Rabbit monoclonal [SP18] to CD79a, prediluted

  • Host species

    Rabbit

  • Specificity

    We have data to indicate that this antibody may not cross react with Opossum. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Pig, Monkey

  • Immunogen

    Synthetic peptide within Human CD79a aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P11912

  • Epitope

    N terminus.

  • Positive control

    • Human tonsil.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready to use for 10 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Notes
IHC-P
1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready to use for 10 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function

    Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.

  • Tissue specificity

    B-cells.

  • Involvement in disease

    Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

  • Cellular localization

    Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt

  • Database links

    • Alternative names

      • B lymphocyte-specific MB1 protein antibody
      • B-cell antigen receptor complex-associated protein alpha chain antibody
      • CD 79a antibody
      • CD79a antibody
      • CD79a antigen (immunoglobulin-associated alpha) antibody
      • CD79A antigen antibody
      • CD79a molecule, immunoglobulin-associated alpha antibody
      • CD79A_HUMAN antibody
      • Ig alpha antibody
      • Ig-alpha antibody
      • IGA antibody
      • IgM-alpha antibody
      • Immunoglobulin-associated alpha antibody
      • Ly54 antibody
      • MB-1 membrane glycoprotein antibody
      • MB1 antibody
      • Membrane-bound immunoglobulin-associated protein antibody
      • Surface IgM-associated protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18], prediluted (ab27607)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18], prediluted (ab27607)
      Ab27607, prediluted, staining CD79a in formalin fixed paraffin embedded human tonsil sections by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18], prediluted (ab27607)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18], prediluted (ab27607)
      Ab27607, prediluted, staining CD79a in formalin fixed paraffin embedded human B cell lymphoma sections by Immunohistochemistry.

    References (0)

    Publishing research using ab27607? Please let us know so that we can cite the reference in this datasheet.

    ab27607 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com