Recombinant Anti-CD79b antibody [CB3-1] (ab130422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [CB3-1] to CD79b
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD79b antibody [CB3-1]
See all CD79b primary antibodies -
Description
Mouse monoclonal [CB3-1] to CD79b -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human peripheral blood; Ramos cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 27th October 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CB3-1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. -
Tissue specificity
B-cells. -
Involvement in disease
Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Post-translational
modificationsPhosphorylated on tyrosine upon B-cell activation. -
Cellular localization
Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 974 Human
- Omim: 147245 Human
- SwissProt: P40259 Human
- Unigene: 89575 Human
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Alternative names
- AGM6 antibody
- B cell antigen receptor complex associated protein beta chain antibody
- B cell specific glycoprotein B29 antibody
see all
Images
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Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells labeling CD79b with ab130422 at 1/50 (10 μg/mL) dilution (Red) compared with an Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.
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Flow cytometry staining of human PBMC for CD79b with ab130422 at 1/50 dilution (10 μg/mL) (right) or an isotype control (left). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab130422 has been referenced in 2 publications.
- Matutes E New additions to antibody panels in the characterisation of chronic lymphoproliferative disorders. J Clin Pathol 55:180-3 (2002). PubMed: 11896067
- Nakamura T et al. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A 89:8522-6 (1992). PubMed: 1382292