Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6860] to CD79b
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-CD79b antibody [EPR6860]
See all CD79b primary antibodies
DescriptionRabbit monoclonal [EPR6860] to CD79b
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human CD79b (UniProt ID: P40259).
- Daudi, Namalwa, Ramos, and Raji cell lysates, Ramos cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.90 x 10 -9 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35-40 kDa (predicted molecular weight: 26 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionRequired in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Involvement in diseaseDefects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain.
modificationsPhosphorylated on tyrosine upon B-cell activation.
Cellular localizationCell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
- Information by UniProt
- AGM6 antibody
- B cell antigen receptor complex associated protein beta chain antibody
- B cell specific glycoprotein B29 antibody
All lanes : Anti-CD79b antibody [EPR6860] (ab134103) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Namalwa cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 35-40 kDa why is the actual band size different from the predicted?
Flow Cytometry analysis of Raji cells labeling CD79b with ab134103 at 1/20 dilution (red). A rabbit IgG was used as negative control (green).
Immunofluorescence analysis of CD79b in Ramos cells, using ab134103 antibody at a dilution of 1/250.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134103 has been referenced in 1 publication.
- Yoo EJ et al. Identification of a secondary promoter within the human B cell receptor component gene hCD79b. J Biol Chem 288:18353-65 (2013). WB . PubMed: 23649625