Overview

  • Product name

    Anti-CD79b antibody [EPR6860]
    See all CD79b primary antibodies
  • Description

    Rabbit monoclonal [EPR6860] to CD79b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CD79b (UniProt ID: P40259).

  • Positive control

    • Daudi, Namalwa, Ramos, and Raji cell lysates, Ramos cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.90 x 10 -9 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6860
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35-40 kDa (predicted molecular weight: 26 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
    • Tissue specificity

      B-cells.
    • Involvement in disease

      Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine upon B-cell activation.
    • Cellular localization

      Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
    • Information by UniProt
    • Database links

    • Alternative names

      • AGM6 antibody
      • B cell antigen receptor complex associated protein beta chain antibody
      • B cell specific glycoprotein B29 antibody
      • B-cell antigen receptor complex-associated protein beta chain antibody
      • B-cell-specific glycoprotein B29 antibody
      • B29 antibody
      • B29/Ig-beta/CD79b antibody
      • CD 79b antibody
      • CD79b antibody
      • CD79b antigen (immunoglobulin associated beta) antibody
      • CD79b antigen antibody
      • CD79b molecule antibody
      • CD79b molecule immunoglobulin associated beta antibody
      • CD79b protein antibody
      • CD79B_HUMAN antibody
      • Ig beta antibody
      • Ig-beta antibody
      • IGB antibody
      • Igbeta antibody
      • Immunoglobulin associated B29 antibody
      • Immunoglobulin associated B29 protein antibody
      • Immunoglobulin associated beta antibody
      • Immunoglobulin associated protein antibody
      • Immunoglobulin-associated B29 protein antibody
      • MGC108607 antibody
      see all

    Images

    • All lanes : Anti-CD79b antibody [EPR6860] (ab134103) at 1/1000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Namalwa cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 35-40 kDa
      why is the actual band size different from the predicted?

    • Immunofluorescence analysis of CD79b in Ramos cells, using ab134103 antibody at a dilution of 1/250.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Yoo EJ  et al. Identification of a secondary promoter within the human B cell receptor component gene hCD79b. J Biol Chem 288:18353-65 (2013). WB . Read more (PubMed: 23649625) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134103.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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