Recombinant
RabMAb

Recombinant Anti-CD79b antibody [EPR6861] (ab134147)

Overview

  • Product name

    Anti-CD79b antibody [EPR6861]
    See all CD79b primary antibodies
  • Description

    Rabbit monoclonal [EPR6861] to CD79b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminal in Human CD79b (P40259).

  • Positive control

    • Daudi, Namalwa, and Raji cell lysates, Human spleen and tonsil tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 4.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6861
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
  • Tissue specificity

    B-cells.
  • Involvement in disease

    Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine upon B-cell activation.
  • Cellular localization

    Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGM6 antibody
    • B cell antigen receptor complex associated protein beta chain antibody
    • B cell specific glycoprotein B29 antibody
    • B-cell antigen receptor complex-associated protein beta chain antibody
    • B-cell-specific glycoprotein B29 antibody
    • B29 antibody
    • B29/Ig-beta/CD79b antibody
    • CD 79b antibody
    • CD79b antibody
    • CD79b antigen (immunoglobulin associated beta) antibody
    • CD79b antigen antibody
    • CD79b molecule antibody
    • CD79b molecule immunoglobulin associated beta antibody
    • CD79b protein antibody
    • CD79B_HUMAN antibody
    • Ig beta antibody
    • Ig-beta antibody
    • IGB antibody
    • Igbeta antibody
    • Immunoglobulin associated B29 antibody
    • Immunoglobulin associated B29 protein antibody
    • Immunoglobulin associated beta antibody
    • Immunoglobulin associated protein antibody
    • Immunoglobulin-associated B29 protein antibody
    • MGC108607 antibody
    see all

Images

  • All lanes : Anti-CD79b antibody [EPR6861] (ab134147) at 1/1000 dilution

    Lane 1 : Daudi cell lysates
    Lane 2 : Namalwa cell lysates
    Lane 3 : Raji cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunofluorescence staining of Daudi cells with purified ab134147 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Immunohistochemical analysis of paraffin embedded Human spleen tissue labelled with ab134147 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab134147 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab134147 showing positive staining in Diffuse B-cell lymphoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab134147 showing negative staining in Normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab134147 showing negative staining in Skeletal muscle tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab134147 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Yoo EJ  et al. Identification of a secondary promoter within the human B cell receptor component gene hCD79b. J Biol Chem 288:18353-65 (2013). WB . Read more (PubMed: 23649625) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Thymus)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
45 µg
Treatment
Noninfected controls and virally infected samples
Specification
Thymus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jun 13 2018

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