Key features and details
- Mouse monoclonal [3-298] to CD8 alpha (Biotin)
- Suitable for: Flow Cyt, IP, IHC-Fr
- Reacts with: Chicken, Turkey
- Conjugation: Biotin
- Isotype: IgG2b
Product nameAnti-CD8 alpha antibody [3-298] (Biotin)
See all CD8 alpha primary antibodies
DescriptionMouse monoclonal [3-298] to CD8 alpha (Biotin)
Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
Species reactivityReacts with: Chicken, Turkey
The details of the immunogen for this antibody are not available.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab25167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use with acetone fixed tissues, at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Purification of CD8+ T cells by positive cell sorting: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
ab25167 has not yet been referenced specifically in any publications.