Anti-CD8 alpha antibody [53-6.7] (ab25478)

Reviews (2)Q&A (2)References (6)

Overview

  • Product name

    Anti-CD8 alpha antibody [53-6.7]
    See all CD8 alpha primary antibodies

  • Description

    Rat monoclonal [53-6.7] to CD8 alpha

  • Host species

    Rat

  • Specificity

    ab25478 recognises a chain of the murine CD8 heterodimer.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, Functional Studiesmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...

  • Purity

    Affinity purified

  • Primary antibody notes

    This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

  • Clonality

    Monoclonal

  • Clone number

    53-6.7

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration. Acetone fixed.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
(In vivo and in vitro depletion of CD8a+ cells): Use at an assay dependent dilution.
Fractionation of CD8a+ T cells: Use at an assay dependent dilution.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • alpha polypeptide (p32) antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly3 antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Images

    • Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)
      Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)

      Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab25478 at 1 μg/106 cells, and Rat Anti-Mouse CD4-UNLB, followed by Mouse Anti-Rat IgG2a-AF647 and Mouse Anti-Rat IgG2b-AF488.

    • Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [53-6.7] (ab25478)
      Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [53-6.7] (ab25478)Image courtesy of an anonymous Abreview
      ab25478 staining CD8 alpha in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/250 dilution.

      See Abreview

    References

    This product has been referenced in:

    See all 6 Publications for this product

    Publishing research using ab25478? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Question

    Regarding the bulk order of anti-CD8 (53-6.7) (Product code ab25478) and anti-CD4 (GK1.5) (Product code ab25475) antibodies.

    Both antibodies will be used for CD8 and CD4 T cell depletions respectively in BALB/c mice. In order to request the amount of each antibody required for my experiment I need the depletion protocol from Abcam for each antibody.

    Looking forward for your response.

    Read More
    Answer

    I can confirm that these two antibodies ab25478 and ab25475 are tested externally in functional studies.

    There are publications where these clones have been tested in this application. I have attached thse for your review and hope this will be helpful.

    The protocol referenced for CD4 antibody [GK1.5] can be found in the following book. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.

    I hope this information will be helpful. Please do not hesitae to let me know if you have any questions or if I may assist you further.

    Read More

    Question

    Dear abcam support desk, Thankyou so much for your fast response. I’mwondering if you still sell this item ab52854 because this antibody works great onour mouse section. Ifnot, do you have any recommendation? Let me know. Thank You for your help.  

    Read More
    Answer

    Thank you for contacting us. We have many other products that works well on mouse tissues and are fully tested. The catalogue numbers are Un conjugated anti CD8 antibodies ab22378 ab25478 ab3081 ab108292 Conjugated anti CD8 antibodies ab25294 ab25676 ab60077 ab25499 ab25498 ab39853 These antibodies will work on mouse section in the applications as recommended on datasheet. Please check the datasheets and buy the antibody as per your requirements. All antibodies are equally good and are fully guaranteed under Abpromise to work with mouse samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD8 alpha antibody [53-6.7]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (bone marrow)
    Specification
    bone marrow
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 19 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-CD8 alpha antibody [53-6.7]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 30 2010

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