Anti-CD8 alpha antibody [53-6.7] (ab25478)
![Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)](https://www.abcam.com/ps/products/25/ab25478/Images/ab25478-271429-anti-cd8-alpha-antibody-53-67-flow-cytometry.jpg "Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)")
Key features and details
- Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD8 alpha antibody [53-6.7]
See all CD8 alpha primary antibodies -
Description
Rat monoclonal [53-6.7] to CD8 alpha -
Host species
Rat -
Specificity
ab25478 recognises a chain of the murine CD8 heterodimer. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD8 alpha. Spleen cells or thymocyte membranes
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General notes
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. -
Clonality
Monoclonal -
Clone number
53-6.7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
- APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 2µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 2µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)
Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab25478 at 1 μg/106 cells, and Rat Anti-Mouse CD4-UNLB, followed by Mouse Anti-Rat IgG2a-AF647 and Mouse Anti-Rat IgG2b-AF488.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab25478 has been referenced in 10 publications.
- Abu Tayeh A et al. Psoriasis-like skin disorder in transgenic mice expressing a RIG-I Singleton-Merten syndrome variant. Int Immunol 33:211-224 (2021). PubMed: 33119735
- Balam S et al. Cross-presentation of dead-cell-associated antigens by DNGR-1+ dendritic cells contributes to chronic allograft rejection in mice. Eur J Immunol 50:2041-2054 (2020). PubMed: 32640051
- Yoo B et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). PubMed: 30886310
- Ritsma L et al. Integrin ß1 activation induces an anti-melanoma host response. PLoS One 12:e0175300 (2017). IHC, ICC/IF ; Mouse, Human . PubMed: 28448494
- Schütte-Nütgen K et al. Divergent Function of Programmed Death-Ligand 1 in Donor Tissue versus Recipient Immune System in a Murine Model of Bronchiolitis Obliterans. Am J Pathol 187:1368-1379 (2017). PubMed: 28427861
- Guan C et al. Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxid Med Cell Longev 2017:9303054 (2017). PubMed: 29456788
- Roffê E et al. Trypanosoma cruzi Causes Paralyzing Systemic Necrotizing Vasculitis Driven by Pathogen-Specific Type I Immunity in Mice. Infect Immun 84:1123-1136 (2016). PubMed: 26857570
- Byrne KT & Vonderheide RH CD40 Stimulation Obviates Innate Sensors and Drives T Cell Immunity in Cancer. Cell Rep 15:2719-32 (2016). PubMed: 27292635
- Gu ME et al. Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata. Dongwuxue Yanjiu 35:249-55 (2014). PubMed: 25017742
- Brunner SM et al. IL-13 signaling via IL-13Ra2 triggers TGF-ß1-dependent allograft fibrosis. Transplant Res 2:16 (2013). IHC . PubMed: 24143891