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    cd8-alpha-antibody-53-67-ab25478.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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Anti-CD8 alpha antibody [53-6.7] (ab25478)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (8)

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Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)

    Key features and details

    • Rat monoclonal [53-6.7] to CD8 alpha
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-CD8 alpha antibody [53-6.7]
      See all CD8 alpha primary antibodies
    • Description

      Rat monoclonal [53-6.7] to CD8 alpha
    • Host species

      Rat
    • Specificity

      ab25478 recognises a chain of the murine CD8 heterodimer.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to CD8 alpha. Spleen cells or thymocyte membranes

    • General notes


      This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 8.20
      Constituent: 100% Borate buffered saline
    • Concentration information loading...
    • Purity

      Affinity purified
    • Primary antibody notes

      This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
    • Clonality

      Monoclonal
    • Clone number

      53-6.7
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • Cytotoxic Cells
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Cancer
      • Tumor immunology
      • CD markers

    Associated products

    • Alternative Versions

      • Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
      • PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
      • PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
      • APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
      • FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
      • PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
      • Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
      • PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
      • APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
      • PE/Cy5® Anti-CD8 alpha antibody [53-6.7] (ab95588)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
    • Recombinant Protein

      • Recombinant Human CD8 alpha protein (ab114161)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25478 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 2µg for 106 cells.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 2µg for 106 cells.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in disease

      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P01732 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12525 Mouse
      • SwissProt: P01731 Mouse
      • Unigene: 1858 Mouse
      • Alternative names

        • alpha polypeptide (p32) antibody
        • CD_antigen=CD8a antibody
        • CD8 antibody
        • CD8 antigen alpha polypeptide antibody
        • CD8 antigen alpha polypeptide (p32) antibody
        • CD8 antigen, alpha polypeptide (p32) antibody
        • CD8a antibody
        • CD8A antigen antibody
        • CD8A molecule antibody
        • CD8A_HUMAN antibody
        • Leu2 antibody
        • Leu2 T lymphocyte antigen antibody
        • Ly 2 antibody
        • Ly 35 antibody
        • Ly B antibody
        • Ly2 antibody
        • Ly3 antibody
        • Ly35 antibody
        • LyB antibody
        • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
        • LYT3 antibody
        • MAL antibody
        • OKT8 T cell antigen antibody
        • OTTHUMP00000160760 antibody
        • OTTHUMP00000160764 antibody
        • OTTHUMP00000203528 antibody
        • OTTHUMP00000203721 antibody
        • p32 antibody
        • T cell antigen Leu2 antibody
        • T cell co receptor antibody
        • T lymphocyte differentiation antigen T8/Leu 2 antibody
        • T-cell surface glycoprotein CD8 alpha chain antibody
        • T-cell surface glycoprotein Lyt 2 antibody
        • T-lymphocyte differentiation antigen T8/Leu-2 antibody
        • T8 T cell antigen antibody
        • T8/Leu-2 T-lymphocyte differentiation antigen antibody
        see all

      Images

      • Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)
        Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)

        Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab25478 at 1 μg/106 cells, and Rat Anti-Mouse CD4-UNLB, followed by Mouse Anti-Rat IgG2a-AF647 and Mouse Anti-Rat IgG2b-AF488.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (8)

      Publishing research using ab25478? Please let us know so that we can cite the reference in this datasheet.

      ab25478 has been referenced in 8 publications.

      • Yoo B  et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). PubMed: 30886310
      • Ritsma L  et al. Integrin ß1 activation induces an anti-melanoma host response. PLoS One 12:e0175300 (2017). IHC, ICC/IF ; Mouse, Human . PubMed: 28448494
      • Schütte-Nütgen K  et al. Divergent Function of Programmed Death-Ligand 1 in Donor Tissue versus Recipient Immune System in a Murine Model of Bronchiolitis Obliterans. Am J Pathol 187:1368-1379 (2017). PubMed: 28427861
      • Guan C  et al. Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxid Med Cell Longev 2017:9303054 (2017). PubMed: 29456788
      • Roffê E  et al. Trypanosoma cruzi Causes Paralyzing Systemic Necrotizing Vasculitis Driven by Pathogen-Specific Type I Immunity in Mice. Infect Immun 84:1123-1136 (2016). PubMed: 26857570
      • Byrne KT & Vonderheide RH CD40 Stimulation Obviates Innate Sensors and Drives T Cell Immunity in Cancer. Cell Rep 15:2719-32 (2016). PubMed: 27292635
      • Gu ME  et al. Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata. Dongwuxue Yanjiu 35:249-55 (2014). PubMed: 25017742
      • Brunner SM  et al. IL-13 signaling via IL-13Ra2 triggers TGF-ß1-dependent allograft fibrosis. Transplant Res 2:16 (2013). IHC . PubMed: 24143891

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Question

      Regarding the bulk order of anti-CD8 (53-6.7) (Product code ab25478) and anti-CD4 (GK1.5) (Product code ab25475) antibodies.

      Both antibodies will be used for CD8 and CD4 T cell depletions respectively in BALB/c mice. In order to request the amount of each antibody required for my experiment I need the depletion protocol from Abcam for each antibody.

      Looking forward for your response.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 19 2013

      Answer

      I can confirm that these two antibodies ab25478 and ab25475 are tested externally in functional studies.

      There are publications where these clones have been tested in this application. I have attached thse for your review and hope this will be helpful.

      The protocol referenced for CD4 antibody [GK1.5] can be found in the following book. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.

      I hope this information will be helpful. Please do not hesitae to let me know if you have any questions or if I may assist you further.

      Read More

      Abcam Scientific Support

      Answered on Apr 19 2013

      Question

      Dear abcam support desk, Thankyou so much for your fast response. I’mwondering if you still sell this item ab52854 because this antibody works great onour mouse section. Ifnot, do you have any recommendation? Let me know. Thank You for your help.  

      Read More

      Abcam community

      Verified customer

      Asked on Aug 22 2011

      Answer

      Thank you for contacting us. We have many other products that works well on mouse tissues and are fully tested. The catalogue numbers are Un conjugated anti CD8 antibodies ab22378 ab25478 ab3081 ab108292 Conjugated anti CD8 antibodies ab25294 ab25676 ab60077 ab25499 ab25498 ab39853 These antibodies will work on mouse section in the applications as recommended on datasheet. Please check the datasheets and buy the antibody as per your requirements. All antibodies are equally good and are fully guaranteed under Abpromise to work with mouse samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Aug 22 2011

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD8 alpha antibody [53-6.7]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (bone marrow)
      Specification
      bone marrow
      Fixative
      Methanol
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 19 2011

      Immunohistochemistry (Frozen sections) abreview for Anti-CD8 alpha antibody [53-6.7]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (spleen)
      Specification
      spleen
      Fixative
      Acetone
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 30 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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