Anti-CD8 alpha antibody [53-6.7] (ab25478)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-CD8 alpha antibody [53-6.7]
See all CD8 alpha primary antibodies -
DescriptionRat monoclonal [53-6.7] to CD8 alpha
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Host speciesRat
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Specificityab25478 recognises a chain of the murine CD8 heterodimer.
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
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Species reactivityReacts with: Mouse
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Immunogen
The details of the immunogen for this antibody are not available.
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General notes
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
PurityAffinity purified
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Primary antibody notesThis antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
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ClonalityMonoclonal
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Clone number53-6.7
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
- Anti-CD8 alpha antibody [53-6.7] (Alexa Fluor® 488) (ab187774)
- Anti-CD8 alpha antibody [53-6.7] (PerCP/Cy5.5®) (ab210236)
- Anti-CD8 alpha antibody [53-6.7] (Biotin) (ab25176)
- Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin -Texas Red®) (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy7 ®) (ab25456)
- Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin) (ab25498)
- Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin) (ab25499)
- Anti-CD8 alpha antibody [53-6.7] (FITC) (ab25676)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy5.5 ®) (ab37928)
- Anti-CD8 alpha antibody [53-6.7] (Cy5 ®) (ab39850)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy7 ®) (ab39853)
- Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy5.5 ® ) (ab82005)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588)
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab25478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use 2µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. Acetone fixed. | |
| IP | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent concentration. (In vivo and in vitro depletion of CD8a+ cells): Use at an assay dependent dilution.Fractionation of CD8a+ T cells: Use at an assay dependent dilution. |
Target
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FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
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Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
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Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
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Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD8 antibody
- CD8 antigen alpha polypeptide antibody
see all
Images
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![Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)](https://www.abcam.com/ps/products/25/ab25478/Images/ab25478-271429-ab25478FC.jpg)
Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (ab25478)Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab25478 at 1 μg/106 cells, and Rat Anti-Mouse CD4-UNLB, followed by Mouse Anti-Rat IgG2a-AF647 and Mouse Anti-Rat IgG2b-AF488.
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![Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [53-6.7] (ab25478)](https://www.abcam.com/ps/products/25/ab25478/Images/CD8-alpha-Primary-antibodies-ab25478-2.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [53-6.7] (ab25478)Image courtesy of an anonymous Abreviewab25478 staining CD8 alpha in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/250 dilution.
Protocols
References
This product has been referenced in:
- Ritsma L et al. Integrin ß1 activation induces an anti-melanoma host response. PLoS One 12:e0175300 (2017). IHC, ICC/IF ; Mouse, Human . Read more (PubMed: 28448494) »
- Gu ME et al. Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata. Dongwuxue Yanjiu 35:249-55 (2014). Read more (PubMed: 25017742) »
