Overview

  • Product name

    Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy5.5 ® )
    See all CD8 alpha primary antibodies
  • Description

    Rat monoclonal [53-6.7] to CD8 alpha (Allophycocyanin/Cy5.5 ® )
  • Host species

    Rat
  • Conjugation

    Allophycocyanin/Cy5.5 ® . Ex: 650nm, Em: 690nm
  • Tested applications

    Suitable for: Flow Cyt, IHC-Fr, IP, Depletion, Functional Studiesmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Mouse mesenteric lymph node cells.
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 16% Sucrose, PBS

    Also contains a stabilizing agent.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Primary antibody notes

    In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
  • Clonality

    Monoclonal
  • Clone number

    53-6.7
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Depletion Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Fractionation of CD8a+ T cells.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Images

  • Flow cytometry analysis of BALB/c mouse splenocytes labelling CD8 alpha with ab82005 at 0.1 µg/106cells and CD19 with rat anti-mouse CD19-FITC.

References

This product has been referenced in:

  • Takahashi K  et al. CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A 89:5557-61 (1992). Depletion ; Mouse . Read more (PubMed: 1608966) »
  • Ledbetter JA  et al. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152:280-95 (1980). Flow Cyt ; Mouse . Read more (PubMed: 6156984) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab82005.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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