Key features and details
- Rat monoclonal [53-6.7] to CD8 alpha (APC/Cy5.5®)
- Suitable for: Flow Cyt, IHC-Fr, IP, Depletion, Functional Studies
- Reacts with: Mouse
- Conjugation: APC/Cy5.5®. Ex: 650nm, Em: 690nm
- Isotype: IgG2a
Product nameAnti-CD8 alpha antibody [53-6.7] (APC/Cy5.5®)
See all CD8 alpha primary antibodies
DescriptionRat monoclonal [53-6.7] to CD8 alpha (APC/Cy5.5®)
ConjugationAPC/Cy5.5®. Ex: 650nm, Em: 690nm
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Depletion, Functional Studiesmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- Mouse mesenteric lymph node cells.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 16% Sucrose, PBS
Also contains a stabilizing agent.
Concentration information loading...
Primary antibody notesIn vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Light chain typekappa
- Anti-CD8 alpha antibody [53-6.7] (Biotin) (ab25176)
- Anti-CD8 alpha antibody [53-6.7] (PE/Texas Red®) (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- Anti-CD8 alpha antibody [53-6.7] (PE) (ab25498)
- Anti-CD8 alpha antibody [53-6.7] (APC) (ab25499)
- Anti-CD8 alpha antibody [53-6.7] (FITC) (ab25676)
- Anti-CD8 alpha antibody [53-6.7] (PE) (ab272343)
- Anti-CD8 alpha antibody [53-6.7] (Cy5 ®) (ab39850)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy7®) (ab39853)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588)
Our Abpromise guarantee covers the use of ab82005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Depletion||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
Fractionation of CD8a+ T cells.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
ab82005 has been referenced in 3 publications.
- Takahashi K et al. CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A 89:5557-61 (1992). Depletion ; Mouse . PubMed: 1608966
- Ledbetter JA et al. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152:280-95 (1980). Flow Cyt ; Mouse . PubMed: 6156984
- Ledbetter JA & Herzenberg LA Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47:63-90 (1979). Flow Cyt, IP ; Mouse . PubMed: 398327