Overview

  • Product name
    Anti-CD8 alpha antibody [53-6.7] (Biotin)
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [53-6.7] to CD8 alpha (Biotin)
  • Host species
    Rat
  • Conjugation
    Biotin
  • Specificity
    ab25176 recognises a chain of the murine CD8 heterodimer, Mr 32-34 kDa.
  • Tested applications
    Suitable for: Functional Studies, Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Avoid freeze / thaw cycle. Do Not Freeze.
  • Storage buffer
    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity
    Affinity purified
  • Primary antibody notes
    In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
  • Clonality
    Monoclonal
  • Clone number
    53-6.7
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

In vivo and in vitro depletion of CD8 alpha+ cells. Fractionation of CD8 alpha+ T cells.

Flow Cyt Use 2µl for 106 cells.

ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Images

  •  

     

    Flow cytometry analysis showing C57Bl/6 splenocytes stained with ab25176 (solid line). Isotype control antibody was Rat Ig2a Biotin (dashed line).

References

ab25176 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for contacting us.
I have received following reply from my colleague when asked for immunogen;
"The immunogen used to produce clone 53-6.7 was mouse thymus or spleen. Generation of this clone is described in the below reference article. At this time, we do not have any information regarding cross reactivity with Drosophila proteins. We apologize for any inconvenience.

Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63."
Assuming that the immunogen is from CD8 alpha chain, I blasted it and found no homology with any of Drosophila protein. So I am sorry I will not be able to provide you any definite answer in this case.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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