Product nameAnti-CD8 alpha antibody [53-6.7] (Biotin)
See all CD8 alpha primary antibodies
DescriptionRat monoclonal [53-6.7] to CD8 alpha (Biotin)
Specificityab25176 recognises a chain of the murine CD8 heterodimer, Mr 32-34 kDa.
Tested applicationsSuitable for: Functional Studies, Flow Cyt, IP, IHC-Frmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Avoid freeze / thaw cycle. Do Not Freeze.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Primary antibody notesIn vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Light chain typekappa
- Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin -Texas Red®) (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin) (ab25498)
- Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin) (ab25499)
- Anti-CD8 alpha antibody [53-6.7] (FITC) (ab25676)
- Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin) (ab272343)
- Anti-CD8 alpha antibody [53-6.7] (Cy5 ®) (ab39850)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy7 ®) (ab39853)
- Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy5.5 ® ) (ab82005)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588)
Our Abpromise guarantee covers the use of ab25176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
In vivo and in vitro depletion of CD8 alpha+ cells. Fractionation of CD8 alpha+ T cells.
|Flow Cyt||Use 2µl for 106 cells.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD8 antibody
- CD8 antigen alpha polypeptide antibody
ab25176 has not yet been referenced specifically in any publications.