Key features and details
- Rat monoclonal [53-6.7] to CD8 alpha (PE/Cy5®)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2a
Product nameAnti-CD8 alpha antibody [53-6.7] (PE/Cy5®)
See all CD8 alpha primary antibodies
DescriptionRat monoclonal [53-6.7] to CD8 alpha (PE/Cy5®)
ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Spleen cells of Mouse origin
- BALB/c splenocytes; mouse thymocyte and splenocyte suspensions
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate, 0.1% Gelatin
Concentration information loading...
Light chain typekappa
- Anti-CD8 alpha antibody [53-6.7] (Biotin) (ab25176)
- Anti-CD8 alpha antibody [53-6.7] (PE/Texas Red®) (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- Anti-CD8 alpha antibody [53-6.7] (PE) (ab25498)
- Anti-CD8 alpha antibody [53-6.7] (APC) (ab25499)
- Anti-CD8 alpha antibody [53-6.7] (FITC) (ab25676)
- Anti-CD8 alpha antibody [53-6.7] (PE) (ab272343)
- Anti-CD8 alpha antibody [53-6.7] (Cy5 ®) (ab39850)
- Anti-CD8 alpha antibody [53-6.7] (PE/Cy7®) (ab39853)
- Anti-CD8 alpha antibody [53-6.7] (APC/Cy5.5®) (ab82005)
Our Abpromise guarantee covers the use of ab95588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Staining the cell sample in a final volume of 100 µL is recommended.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
ab95588 has been referenced in 3 publications.
- Yang Z et al. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation 114:2056-64 (2006). PubMed: 17060376
- Taylor JL et al. Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA. Infect Immun 73:5189-93 (2005). PubMed: 16041037
- Grabbe S et al. Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest 109:183-92 (2002). PubMed: 11805130