Key features and details
- Rabbit polyclonal to CD8 alpha
- Suitable for: IHC-Fr, Flow Cyt, ICC/IF, WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD8 alpha antibody
See all CD8 alpha primary antibodies
DescriptionRabbit polyclonal to CD8 alpha
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- HuT78 cells, Human peripheral blood lymphocytes and Human Tonsil.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab187366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 - 2 µg/ml.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 26 kDa.|
|IP||Use at 2-4 µg/mg of lysate.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
ab187366 has not yet been referenced specifically in any publications.