Recombinant
RabMAb

Recombinant Anti-CD8 alpha antibody [EP1150] (ab108343)

Overview

  • Product name
    Anti-CD8 alpha antibody [EP1150]
    See all CD8 alpha primary antibodies
  • Description
    Rabbit monoclonal [EP1150] to CD8 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD8 alpha aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P01732

  • Positive control
    • Jurkat whole cell lysate (ab7899) can be used as a positive control in WB. IHC-P: Human tonsil tissue. ICC/IF: U-937 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP1150
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Images

  • Flow cytometric analysis of paraformaldehyde-fixed human peripheral blood mononuclear cells (PBMC) labeling CD8 alpha with ab108343 at 1/40 dilution, followed by F(ab')2 Frag Goat Anti-Rabbit IgG (H+L) Alexa Fluor® 488.

    Gating Strategy: lymphocytes based on FSC vs SSC.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of U-937 cells labelling CD8 alpha with ab108343 at a 1/200 dilution. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were counterstained with ab195889, an Alexa Fluor® 594-conjugated mouse anti-tubulin antibody (1/200). Nuclei counterstained with DAPI (blue).

    Secondary antibody only control: PBS instead of primary antibody.

    Cytoplasmic staining seen in U-937 cells.

  • ab108343 at 1/250 dilution staining CD8 alpha in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

References

This product has been referenced in:
  • Zhou S  et al. Indoleamine 2,3-dioxygenase 1 and Programmed Cell Death-ligand 1 Co-expression Predicts Poor Pathologic Response and Recurrence in Esophageal Squamous Cell Carcinoma after Neoadjuvant Chemoradiotherapy. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 30717285) »
  • Li S  et al. Oxidative stress drives CD8+ T-cell skin trafficking in patients with vitiligo through CXCL16 upregulation by activating the unfolded protein response in keratinocytes. J Allergy Clin Immunol 140:177-189.e9 (2017). Read more (PubMed: 27826097) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization
Yes - 0.5% saponin
Gating Strategy
lymphocytes based on FSC vs SSC
Specification
peripheral blood mononuclear cells (PBMC)
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 13 2016

Answer

Thank you very much for your phone call.

The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

Anti CD3 : ab16669; I can provide Abreview image discount code and will also provide Abpromise guarantee on use for this ab.

Anti FOXP3: ab20034, ab22510 are best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Anti CD4: ab61131, ab101530 and ab108329, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

anti CD8: ab108343, ab123630 and ab86733, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Anti CD1A : ab76531, ab108309 and ab123133 are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Please also note that most of these antibodies are raised in Rabbit so there will be high back ground staining. Our recommendations to reduce the back ground staining are

- Use preadsorbed anti body e.g. ab7132, ab7083, ab

- Use (Fab)2 fragments antibodies e.g. ab6112, ab102296

- Incubate sections with affinity pure Fab fragment anti rabbit antibody before applying primary antibody

Please check the full details by clicking the links;

https://www.abcam.com/index.html?pageconfig=resource&rid=12722
https://www.abcam.com/index.html?pageconfig=resource&rid=12825
https://www.abcam.com/index.html?pageconfig=resource&rid=11465

The abreview discount code details can be found by clicking following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Could you please select the antibodies you are interested in, I will then be happy in creating a discount code for each one of these.

I will look forward to hearing from you soon.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. R Berahovich

Verified customer

Submitted Dec 16 2011

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