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    cd8-alpha-antibody-ep1150y-ab93278.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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RecombinantRabMAb

Recombinant Anti-CD8 alpha antibody [EP1150Y] (ab93278)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (30)

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Immunocytochemistry - Anti-CD8 alpha antibody [EP1150Y] (ab93278)
  • Western blot - Anti-CD8 alpha antibody [EP1150Y] (ab93278)
  • Anti-CD8 alpha antibody [EP1150Y] (ab93278)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1150Y] to CD8 alpha
  • Suitable for: WB, ICC
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Alexa Fluor® 647 Anti-CD8 alpha antibody [EP1150Y] (ab196193)

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Overview

  • Product name

    Anti-CD8 alpha antibody [EP1150Y]
    See all CD8 alpha primary antibodies
  • Description

    Rabbit monoclonal [EP1150Y] to CD8 alpha
  • Host species

    Rabbit
  • Specificity

    The immunogen sequence for this antibody has no significant homology with mouse and rat CD alpha and beta. This product showed specific staining in lymphocytes, although we observed background staining in some epithelial cells tested.
  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human CD8 alpha aa 200 to the C-terminus (C terminal).
    Database link: P01732

  • Positive control

    • WB: THP-1 cell lysate. ICC: MOLT-4 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1150Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-CD8 alpha antibody [EP1150Y] (ab196193)
    • Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)
    • Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)
    • Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • THP-1 whole cell lysate (ab7913)
  • Related Products

    • Anti-IL-15 antibody [EP433Y] (ab40668)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 26 kDa.

For unpurified use at 1/1000 - 1/10000.

ICC
1/500.

For unpurified use at 1/50 - 1/100.

Notes
WB
1/1000. Predicted molecular weight: 26 kDa.

For unpurified use at 1/1000 - 1/10000.

ICC
1/500.

For unpurified use at 1/50 - 1/100.

Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01732 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 925 Human
    • Omim: 186910 Human
    • SwissProt: P01732 Human
    • Unigene: 85258 Human
    • Alternative names

      • alpha polypeptide (p32) antibody
      • CD_antigen=CD8a antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly 2 antibody
      • Ly 35 antibody
      • Ly B antibody
      • Ly2 antibody
      • Ly3 antibody
      • Ly35 antibody
      • LyB antibody
      • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T lymphocyte differentiation antigen T8/Leu 2 antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-cell surface glycoprotein Lyt 2 antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Images

    • Immunocytochemistry - Anti-CD8 alpha antibody [EP1150Y] (ab93278)
      Immunocytochemistry - Anti-CD8 alpha antibody [EP1150Y] (ab93278)

      Immunocytochemistry analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) labeling CD8 alpha with purified ab93278 at 1/250 dilution (9 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.21 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.

    • Western blot - Anti-CD8 alpha antibody [EP1150Y] (ab93278)
      Western blot - Anti-CD8 alpha antibody [EP1150Y] (ab93278)
      Anti-CD8 alpha antibody [EP1150Y] (ab93278) at 1/1000 dilution (unpurified) + THP-1 cell lysate at 10 µg

      Predicted band size: 26 kDa

    • Anti-CD8 alpha antibody [EP1150Y] (ab93278)
      Anti-CD8 alpha antibody [EP1150Y] (ab93278)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (30)

    Publishing research using ab93278? Please let us know so that we can cite the reference in this datasheet.

    ab93278 has been referenced in 30 publications.

    • Zhou C  et al. Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment. Cell Death Differ 28:715-729 (2021). PubMed: 32929219
    • Yang J  et al. Integrated genomic and transcriptomic analysis reveals unique characteristics of hepatic metastases and pro-metastatic role of complement C1q in pancreatic ductal adenocarcinoma. Genome Biol 22:4 (2021). PubMed: 33397441
    • Gao C  et al. CircRNA VIM silence synergizes with sevoflurane to inhibit immune escape and multiple oncogenic activities of esophageal cancer by simultaneously regulating miR-124/PD-L1 axis. Cell Biol Toxicol N/A:N/A (2021). PubMed: 34018092
    • Lei Y  et al. The average copy number variation (CNVA) of chromosome fragments is a potential surrogate for tumor mutational burden in predicting responses to immunotherapy in non-small-cell lung cancer. Clin Transl Immunology 10:e1231 (2021). PubMed: 33425353
    • Zhang A  et al. Lactate-induced M2 polarization of tumor-associated macrophages promotes the invasion of pituitary adenoma by secreting CCL17. Theranostics 11:3839-3852 (2021). PubMed: 33664865
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD8 antibody [EP1150Y]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Mr. 勝啓 平田

    Verified customer

    Submitted Feb 05 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD8 antibody [EP1150Y]

    Below Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (Liver/Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH9
    Permeabilization
    No
    Specification
    Liver/Spleen
    Blocking step
    Casein as blocking agent for 10 minute(s) · Concentration: 1%
    Fixative
    10% Formalin
    Read More

    DR. Inotiv Boulder

    Verified customer

    Submitted Dec 03 2019

    Flow Cytometry abreview for Anti-CD8 antibody [EP1150Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (peripheral blood mononuclear cells (PBMC))
    Permeabilization
    Yes - 0.5% saponin
    Gating Strategy
    lymphocytes based on FSC vs SSC
    Specification
    peripheral blood mononuclear cells (PBMC)
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 13 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD8 antibody [EP1150Y]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Diva Decloaker
    Sample
    Human Tissue sections (Intestine)
    Specification
    Intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 24 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD8 antibody [EP1150Y]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE-Buffer
    Permeabilization
    Yes - DAKO Wash Buffer
    Read More

    MR. Rudolf Jung

    Verified customer

    Submitted Feb 22 2011

    Question

    anti cd4 / cd8 pour faire de l'IHC/IF en rabbit?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2012

    Answer

    Merci de nous avoir contactés.


    Je suis désolée de vous avoir raté aujourd’hui. J'ai essayé de vous rappeler 4 fois aujourd'hui sur votre numéro de téléphone 0492038999, mais je n’ai pas eu de réponse.

    Concernant les anticorps que vous cherchez, je vous recommande Anti-CD8 antibody [EP1150Y], ab93278 (https://www.abcam.com/ab93278) et Anti-CD4 antibody [EPR6855] ab133616 (https://www.abcam.com/ab133616). Ils sont tous les deux des monoclonaux de lapin qui ont été testées en IHC-P chez l’humain.

    En ce moment il y’a un offre où si vous achetez un anticorps primaire, ont vous offre un monoclonal de lapin gratuit. Cette offre n’est que valable jusqu'à la fin du mois.

    Le code est xxxxxxxx que vous n’avez qu’à ajouter sur votre commande pour profiter de cette promotion.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Vous utilisez nos produits? Partagez vos résultats sous forme d'Abreview et recevez en échange des CADEAUX!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 14 2012

    Question

    ich bin an einer immunohistochemischen Färbung von humanem CD8 und humanem FoxP3 (in Mausgewebe) interessiert.



    - Idealerweise mit einen rabbit anti CD-8 Primärantikörper

    - Und FoxP3 mit Alkalischer Phosphatase (o.a.) gelabeltem Sekundärantikörper, um ggf. später ein doublestaining zu machen



    Dazu hätte ich zwei Fragen: Welcher Unterschied besteht zwischen Antikörpern aus Überstanden bzw. aufgereinigten Antikörpern? Gibt es spezielle Vor-oder Nachteile bei den jeweiligen Methoden?



    Würden Sie mir bitte ein Angebot mit jeweils passenden Sekundärantikörper machen?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 23 2012

    Answer

    Vielen Dank für Ihre Anfrage.

    Ich habe Ihnen hier vier Antikörper herausgesucht, die wenig bzw. gar keine Kreuzreaktion mit dem Maus-Protein aufweisen, so dass Sie nur das humane Protein im Mausgewebe detektieren sollten.

    Drei der vier Antikörper stammen aus dem Kaninchen, falls Sie also eine Doppelfärbung durchführen möchten, wurde ich Vorschlagen, einen der Rabbit Antikörper mit einem ungelabelten Sekundär- Antikörper zu blockieren, und dann den sekundären Antikörper mit einem tertiären Antikörper nachzuweisen: zb. im Falle der Kombination aus ab93278 und ab10563.

    Dazu passen wuerden dann die folgenden Antikörper: ab6831 Chicken polyclonal Secondary Antibody to Rabbit IgG - H&L gefolgt von ab97142 Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alkaline Phosphatase), pre-adsorbed ; und ab7084 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed.

    Oder aber Sie benutzen zwei primäre Antikörper aus zwei verschiedenen Species (ab2481 und ab4055).

    Als Sekundär- Antikoerper kommen dann ab7084 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed; und ab6886 Donkey anti-Goat IgG H&L (AP) secondary antibody.

    Bezüglich Ihrer Frage nach dem Aufreinigungsgrad: Der einzige Vorteil eines aufgereinigten Antikörpers ist es, dass Sie eine Isotopenkontrolle berechnen können, da Sie genau wissen, wie viel Antikörper Sie einsetzen. Dies ist aber eigentlich nur in der Flow Zytometrie von Interesse.


    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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