Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (SW480 tumor taken out balb/c nude mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Permeabilization
No
Specification
SW480 tumor taken out balb/c nude mice
Blocking step
Peroxidase Blocking Solution, BLOXALL as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Signalstain ab diluent
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ImmPRESS® HRP Goat Anti-Rabbit IgG Polymer Detecti
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
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Submitted Aug 11 2021